Comparison of methods to enumerate bacteria in dental unit water lines

Millipore HPC samplers are simple, self-contained test devices that can be used by personnel in dental offices who do not have microbiologic training to easily and economically monitor dental unit water quality without laboratory support. This study evaluated the correlation of HPC samplers to R2A a...

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Bibliographic Details
Published in:Current microbiology 1999-02, Vol.38 (2), p.132-134
Main Authors: KARPAY, R. I, PLAMONDON, T. J, MILLS, S. E
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Colony Count, Microbial - methods
Dentistry
Fundamental and applied biological sciences. Psychology
Microbiology
Water Microbiology
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Summary:Millipore HPC samplers are simple, self-contained test devices that can be used by personnel in dental offices who do not have microbiologic training to easily and economically monitor dental unit water quality without laboratory support. This study evaluated the correlation of HPC samplers to R2A agar for enumerating planktonic bacteria in dental unit treatment water. Eight different dental units were sampled. Five replicates were performed for each media at each dilution. The Pearson correlation coefficient between the R2A agar and HPC sampler is 0.89. These data suggest HPC samplers correlate with conventional laboratory-based R2A culture techniques for determining dental unit water line contamination.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900416