Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction

:  As revealed by immunohistochemistry and RT‐QPCR, the focal adhesion protein tensin1 is expressed in cultured human dermal fibroblasts and reduced by 60% after transfection with tensin1 siRNA. Tensin1 silenced fibroblast exhibited a strongly reduced capacity to contract collagen gels. Aged fibrobl...

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Bibliographic Details
Published in:Experimental dermatology 2008-09, Vol.17 (9), p.788-789
Main Authors: Saintigny, Gaëlle, Bernard, François-Xavier, Juchaux, Franck, Pedretti, Nathalie, Mahé, Christian
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cells, Cultured
Collagen - physiology
collagen gel contraction
Dermatology
Fibroblasts - metabolism
human dermal fibroblasts
Humans
Medical sciences
Microfilament Proteins - genetics
Microfilament Proteins - metabolism
RNA, Small Interfering - genetics
Skin Aging
tensin1
Tensins
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Summary::  As revealed by immunohistochemistry and RT‐QPCR, the focal adhesion protein tensin1 is expressed in cultured human dermal fibroblasts and reduced by 60% after transfection with tensin1 siRNA. Tensin1 silenced fibroblast exhibited a strongly reduced capacity to contract collagen gels. Aged fibroblasts, generated with the Hayflick replicative senescence model, exhibit as siRNA silences fibroblasts, a reduced tensin1 expression and an impaired gel contraction capacity. Based on these results, we speculate that in human dermal fibroblasts, tensin1 plays an important role in cell–matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing.
ISSN:0906-6705
1600-0625
DOI:10.1111/j.1600-0625.2008.00707.x