The production of two Th2 cytokines, interleukin-4 and interleukin-10, is controlled independently by locus Cypr1 and by loci Cypr2 and Cypr3, respectively

The strains BALB/cHeA (BALB/c) and STS/A (STS) differ in production of IL-4 and IL-10, two Th2 cytokines, after stimulation of spleen cells with Concanavalin A, STS being a low and BALB/c a high producer. We analyzed the genetic basis of this strain difference using the recombinant congenic (RC) str...

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Bibliographic Details
Published in:Immunogenetics (New York) 1999, Vol.49 (2), p.134-141
Main Authors: Kosarová, M, Havelková, H, Krulová, M, Demant, P, Lipoldová, M
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Chromosome Mapping
Crosses, Genetic
Cypr1 locus
Cypr2 locus
Cypr3 locus
Female
Gene Expression Regulation - immunology
Genetic Linkage - immunology
Genetic Markers - immunology
Genotype
Interleukin-10 - biosynthesis
Interleukin-10 - genetics
Interleukin-4 - biosynthesis
Interleukin-4 - genetics
Male
Mice
Mice, Congenic
Mice, Inbred BALB C
Th2 Cells - metabolism
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Summary:The strains BALB/cHeA (BALB/c) and STS/A (STS) differ in production of IL-4 and IL-10, two Th2 cytokines, after stimulation of spleen cells with Concanavalin A, STS being a low and BALB/c a high producer. We analyzed the genetic basis of this strain difference using the recombinant congenic (RC) strains of the BALB/c-c-STS/Dem (CcS/Dem) series. This series comprises 20 homozygous strains. Each CcS/Dem strain contains a different, random set of approximately 12. 5% genes of the "donor" strain STS and approximately 87.5% of the "background" strain BALB/c. We selected for further analysis the RC strain production intermediate between BALB/c and STS. In (CcS-20xBALB/c)F2 hybrids we found that different loci control expression of IL-4 and IL-10. Cypr1 (cytokine production 1) on chromosome 16 near D16Mit15 controls IL-4 production, whereas the production of IL-10 is influenced by loci Cypr2 near D1Mit14 and D1Mit227 on chromosome 1 and Cypr3 marked by D5Mit20 on chromosome 5. In addition, the relationship between the level of these two cytokines depends on the genotype of the F2 hybrids at a locus cora1 (correlation 1) on chromosome 5. This differential genetic regulation may be relevant for the understanding of biological effects of T-helper cells in mice of different genotypes.
ISSN:0093-7711
1432-1211
DOI:10.1007/s002510050472