Genetic analysis of the cap5 locus of Staphylococcus aureus

Abstract Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-def...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-01, Vol.170 (1), p.97-103
Main Authors: Wann, Elisabeth R., Dassy, Bruno, Fournier, Jean-Michel, Foster, Timothy J.
Format: Article
Language:English
Subjects:
Bacterial Capsules - biosynthesis
Bacterial Capsules - chemistry
Bacterial Capsules - genetics
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Blotting, Southern
cap5 locus
Capsular polysaccharide
Capsular polysaccharides
Carbohydrate Sequence
Complementation
DNA Transposable Elements
DNA, Bacterial
Fundamental and applied biological sciences. Psychology
Gene clusters
Gene expression
Genetic analysis
Genetic Complementation Test
Genetics
Microbiology
Molecular Sequence Data
Mutation
Polysaccharides
Restriction Mapping
Serotypes
Serotyping
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - genetics
Transduction, Genetic
Transposon mutant
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Summary:Abstract Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H–K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13360.x