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Genetic analysis of the cap5 locus of Staphylococcus aureus
Abstract Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-def...
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| Published in: | FEMS microbiology letters 1999-01, Vol.170 (1), p.97-103 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c4347-36fafc1f5eb64bb61cfc051c5f0c7d11024f8a49537b0a3b02358e8ca4316fb3 |
| container_end_page | 103 |
| container_issue | 1 |
| container_start_page | 97 |
| container_title | FEMS microbiology letters |
| container_volume | 170 |
| creator | Wann, Elisabeth R. Dassy, Bruno Fournier, Jean-Michel Foster, Timothy J. |
| description | Abstract
Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H–K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation. |
| doi_str_mv | 10.1111/j.1574-6968.1999.tb13360.x |
| format | article |
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Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H–K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1999.tb13360.x</identifier><identifier>PMID: 9919657</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacterial Capsules - biosynthesis ; Bacterial Capsules - chemistry ; Bacterial Capsules - genetics ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Blotting, Southern ; cap5 locus ; Capsular polysaccharide ; Capsular polysaccharides ; Carbohydrate Sequence ; Complementation ; DNA Transposable Elements ; DNA, Bacterial ; Fundamental and applied biological sciences. Psychology ; Gene clusters ; Gene expression ; Genetic analysis ; Genetic Complementation Test ; Genetics ; Microbiology ; Molecular Sequence Data ; Mutation ; Polysaccharides ; Restriction Mapping ; Serotypes ; Serotyping ; Staphylococcus aureus ; Staphylococcus aureus - classification ; Staphylococcus aureus - genetics ; Transduction, Genetic ; Transposon mutant</subject><ispartof>FEMS microbiology letters, 1999-01, Vol.170 (1), p.97-103</ispartof><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1999</rights><rights>1999 INIST-CNRS</rights><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4347-36fafc1f5eb64bb61cfc051c5f0c7d11024f8a49537b0a3b02358e8ca4316fb3</citedby><cites>FETCH-LOGICAL-c4347-36fafc1f5eb64bb61cfc051c5f0c7d11024f8a49537b0a3b02358e8ca4316fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1680508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9919657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wann, Elisabeth R.</creatorcontrib><creatorcontrib>Dassy, Bruno</creatorcontrib><creatorcontrib>Fournier, Jean-Michel</creatorcontrib><creatorcontrib>Foster, Timothy J.</creatorcontrib><title>Genetic analysis of the cap5 locus of Staphylococcus aureus</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H–K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation.</description><subject>Bacterial Capsules - biosynthesis</subject><subject>Bacterial Capsules - chemistry</subject><subject>Bacterial Capsules - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>cap5 locus</subject><subject>Capsular polysaccharide</subject><subject>Capsular polysaccharides</subject><subject>Carbohydrate Sequence</subject><subject>Complementation</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene clusters</subject><subject>Gene expression</subject><subject>Genetic analysis</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Polysaccharides</subject><subject>Restriction Mapping</subject><subject>Serotypes</subject><subject>Serotyping</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - classification</subject><subject>Staphylococcus aureus - genetics</subject><subject>Transduction, Genetic</subject><subject>Transposon mutant</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqVkVtLxDAQhYMoul5-glBUfGvNNHfFBxFvsOKD-x7SmGCX7rY2Le7-e1u3rCCKmJeQme_kDGcQOgKcQHfOpgkwQWOuuExAKZU0GRDCcbLYQKN1axONMBEyBqzEDtoNYYoxpinm22hbKVCciRG6uHNz1-Q2MnNTLEMeotJHzauLrKlYVJS2_aw8N6Z6XXbP0vYV09auDftoy5siuIPh3kOT25vJ9X08frp7uL4ax5YSKmLCvfEWPHMZp1nGwXqLGVjmsRUvADilXhqqGBEZNiTDKWHSSWsoAe4zsodOV99WdfnWutDoWR6sKwozd2UbNFeMAyj6JwgCUiEl78Djb-C0bOsugKBTAiCYAMI66nxF2boMoXZeV3U-M_VSA9b9HvRU92HrPmzd70EPe9CLTnw4WLTZzL2spUPwXf9k6JtgTeFrM7d5-HLgEjMsO-xyhb3nhVv-YwB9-zhWvQ1b6cu2-kUd_zT-B9uxsvM</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Wann, Elisabeth R.</creator><creator>Dassy, Bruno</creator><creator>Fournier, Jean-Michel</creator><creator>Foster, Timothy J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990101</creationdate><title>Genetic analysis of the cap5 locus of Staphylococcus aureus</title><author>Wann, Elisabeth R. ; Dassy, Bruno ; Fournier, Jean-Michel ; Foster, Timothy J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4347-36fafc1f5eb64bb61cfc051c5f0c7d11024f8a49537b0a3b02358e8ca4316fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacterial Capsules - biosynthesis</topic><topic>Bacterial Capsules - chemistry</topic><topic>Bacterial Capsules - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>cap5 locus</topic><topic>Capsular polysaccharide</topic><topic>Capsular polysaccharides</topic><topic>Carbohydrate Sequence</topic><topic>Complementation</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene clusters</topic><topic>Gene expression</topic><topic>Genetic analysis</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Polysaccharides</topic><topic>Restriction Mapping</topic><topic>Serotypes</topic><topic>Serotyping</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - classification</topic><topic>Staphylococcus aureus - genetics</topic><topic>Transduction, Genetic</topic><topic>Transposon mutant</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wann, Elisabeth R.</creatorcontrib><creatorcontrib>Dassy, Bruno</creatorcontrib><creatorcontrib>Fournier, Jean-Michel</creatorcontrib><creatorcontrib>Foster, Timothy J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wann, Elisabeth R.</au><au>Dassy, Bruno</au><au>Fournier, Jean-Michel</au><au>Foster, Timothy J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of the cap5 locus of Staphylococcus aureus</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>170</volume><issue>1</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Staphylococcus aureus expresses at least eight distinct serotypes of capsular polysaccharide (CP). Gene clusters involved in the expression of serotypes 1, 5 and 8 have been cloned and sequenced. In this report we describe the isolation and analysis of serotype 5 capsular polysaccharide-defective mutants. A naturally occurring cap mutation in the laboratory strains 8325-4 and RN4220 was mapped to the cap5E gene by genetic complementation. The cap5H–K genes were shown to be responsible for CP5 serotype specificity by transduction and complementation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9919657</pmid><doi>10.1111/j.1574-6968.1999.tb13360.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-01, Vol.170 (1), p.97-103 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Bacterial Capsules - biosynthesis Bacterial Capsules - chemistry Bacterial Capsules - genetics Bacterial Proteins - genetics Bacteriology Biological and medical sciences Blotting, Southern cap5 locus Capsular polysaccharide Capsular polysaccharides Carbohydrate Sequence Complementation DNA Transposable Elements DNA, Bacterial Fundamental and applied biological sciences. Psychology Gene clusters Gene expression Genetic analysis Genetic Complementation Test Genetics Microbiology Molecular Sequence Data Mutation Polysaccharides Restriction Mapping Serotypes Serotyping Staphylococcus aureus Staphylococcus aureus - classification Staphylococcus aureus - genetics Transduction, Genetic Transposon mutant |
| title | Genetic analysis of the cap5 locus of Staphylococcus aureus |
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