CMAP : A novel cystatin-like gene involved in liver metastasis

A novel metastasis-associated gene was identified with a differential display system in murine carcinoma cells showing a high rate of metastasis to the liver. A human homologue was also identified using a PCR-based strategy. The protein coded by this gene was named cystatin-like metastasis-associate...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1999, Vol.59 (1), p.151-158
Main Authors: MORITA, M, YOSHIUCHI, N, ARAKAWA, H, NISHIMURA, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Biomarkers, Tumor
Cloning, Molecular
Cystatins - genetics
DNA, Neoplasm - analysis
DNA, Neoplasm - genetics
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Liver Neoplasms - genetics
Liver Neoplasms - secondary
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Mice
Molecular Sequence Data
Neoplasm Metastasis - genetics
Neoplasms, Experimental - genetics
Neoplasms, Experimental - pathology
Sequence Alignment
Tumor Cells, Cultured
Tumors
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Summary:A novel metastasis-associated gene was identified with a differential display system in murine carcinoma cells showing a high rate of metastasis to the liver. A human homologue was also identified using a PCR-based strategy. The protein coded by this gene was named cystatin-like metastasis-associated protein (CMAP) and showed 22.1-28.1% homology to human family 2 cystatins. CMAP mRNA was selectively overexpressed in all murine liver metastatic tumors but not in any pulmonary metastatic tumors examined. Transfection of CMAP antisense DNA into highly metastatic liver cells greatly decreased their metastatic potential and CMAP expression, indicating that CMAP is involved in liver metastatic ability after intravasation of malignant cells. The human homologue of CMAP was found to be expressed in various human cancer cell lines established from malignant tumors. Our discovery of this novel liver metastasis-related gene indicates a new approach to the diagnosis and/or prevention of liver metastasis of human cancer.
ISSN:0008-5472
1538-7445