Phosphorylation and dephosphorylation of the B subunit of DNA polymerase alpha-primase complex in the early embryogenesis of Drosophila

In the early embryos of Drosophila, the B subunit of the DNA polymerase alpha-primase complex was found to migrate more slowly during the first 13 mitotic cycles than that from cycle 14 using SDS-polyacrylamide gel electrophoresis. Lambda phosphatase treatment showed that the reduced migration was c...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-01, Vol.254 (2), p.372-377
Main Authors: Kuroda, K, Ueda, R
Format: Article
Language:English
Subjects:
animal physiology
Animals
arthropods
Blotting, Western
Cell Cycle
DNA Polymerase I - chemistry
DNA Polymerase I - isolation & purification
DNA Polymerase I - metabolism
DNA Primase - chemistry
DNA Primase - isolation & purification
DNA Primase - metabolism
Drosophila melanogaster
Drosophila melanogaster - embryology
Electrophoresis, Polyacrylamide Gel
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - enzymology
Embryo, Nonmammalian - physiology
entomology
Kinetics
Macromolecular Substances
Mitosis
Phosphorylation
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Summary:In the early embryos of Drosophila, the B subunit of the DNA polymerase alpha-primase complex was found to migrate more slowly during the first 13 mitotic cycles than that from cycle 14 using SDS-polyacrylamide gel electrophoresis. Lambda phosphatase treatment showed that the reduced migration was caused by phosphorylation of the B subunit. Detailed analysis using the partially purified B subunit indicated that most of the B subunit until cycle 13 was a phosphorylated form while the B subunit of cycle 14 was a dephosphorylated form.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9944