Effect of the substituted benzaldehyde 12C79 on Cl--dependent K+ influx in human red blood cells

Ouabain- and bumetanide-resistant K+ influx, and haemoglobin (Hb) O2 saturation, were measured in HbA red cells over a range of oxygen tensions (PO2 values) in the presence and absence of 12C79 (5 mM), a substituted benzaldehyde which increases the O2 affinity of Hb. PO2 values for half-maximal O2 s...

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Bibliographic Details
Published in:Pflügers Archiv 1999-02, Vol.437 (3), p.498-500
Main Authors: Gibson, J S, Speake, P F, Ellory, J C
Format: Article
Language:English
Subjects:
Antisickling Agents - pharmacology
Benzaldehydes - pharmacology
Biological Transport - drug effects
Bumetanide - pharmacology
Chlorides - pharmacology
Erythrocytes - drug effects
Erythrocytes - metabolism
Hemoglobins - metabolism
Humans
Ouabain - pharmacology
Oxygen - blood
Potassium - blood
Potassium Chloride - blood
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Summary:Ouabain- and bumetanide-resistant K+ influx, and haemoglobin (Hb) O2 saturation, were measured in HbA red cells over a range of oxygen tensions (PO2 values) in the presence and absence of 12C79 (5 mM), a substituted benzaldehyde which increases the O2 affinity of Hb. PO2 values for half-maximal O2 saturation declined from 29+/-2 mmHg (mean +/-SEM, n=3) in control cells to 7+/-1 mmHg with 12C79. In control cells, Cl--dependent K+ influx (indicative of KCl cotransport activity) was fully O2 dependent, i.e. inactive at low PO2 values. By contrast, in the presence of 12C79, KCl cotransport was largely resistant to inactivation at low PO2 values. Substantial cotransport activity was still present (>60% of that at high PO2 values) in N2, although O2 saturation was low (about 10%). In all cases, Cl--independent K+ influxes were low [
ISSN:0031-6768
1432-2013
DOI:10.1007/s004240050807