Antisense oligodeoxynucleotides of sulfonylurea receptors inhibit ATP-sensitive K+ channels in cultured neonatal rat ventricular cells

To identify the functional sulfonylurea receptor (SUR), a subunit of the adenosine 5'-triphosphate (ATP)-sensitive K+ (KATP) channels, in neonatal rat ventricular cells, such cells in primary culture were treated for 6 days with antisense (AS) oligodeoxynucleotides (ODNs) complementary to the m...

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Bibliographic Details
Published in:Pflügers Archiv 1999-02, Vol.437 (3), p.400-408
Main Authors: Yokoshiki, H, Sunagawa, M, Seki, T, Sperelakis, N
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - pharmacology
Animals
Animals, Newborn
ATP-Binding Cassette Transporters
Cells, Cultured
Diazoxide - pharmacology
Electric Conductivity
Heart - physiology
Oligodeoxyribonucleotides, Antisense - pharmacology
Pinacidil - pharmacology
Potassium
Potassium Channel Blockers
Potassium Channels - genetics
Potassium Channels - physiology
Potassium Channels, Inwardly Rectifying
Rats
Rats, Sprague-Dawley
Receptors, Drug - genetics
Receptors, Drug - physiology
Rodents
Sulfonylurea Receptors
Ventricular Function
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Summary:To identify the functional sulfonylurea receptor (SUR), a subunit of the adenosine 5'-triphosphate (ATP)-sensitive K+ (KATP) channels, in neonatal rat ventricular cells, such cells in primary culture were treated for 6 days with antisense (AS) oligodeoxynucleotides (ODNs) complementary to the mRNA for SURs. For quantification, single-channel (inside-out patches) and whole-cell currents were measured using the patch-clamp technique. The maximal KATP currents (at 0 mV) induced by metabolic inhibition were 48.9+/-2.8 pA/pF in control (n=48), 34.3+/-3.5 pA/pF in AS-SUR1 (n=21, P
ISSN:0031-6768
1432-2013
DOI:10.1007/s004240050794