Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals

A reversed-phase ion-pair high-performance liquid chromatografic method (RP-IPC) was developed to assay some water-soluble vitamins in solution dosage forms. Vitamins of the B-group B 1, B 2, B 3, and B 6, including vitamin C were determined in Oligovit ® coated tablets. In Beviplex ® coated tablets...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1999, Vol.18 (6), p.999-1004
Main Authors: Ivanović, D, Popović, A, Radulović, D, Medenica, M
Format: Article
Language:English
Subjects:
Analysis
Ascorbic Acid - analysis
Biological and medical sciences
Chromatography, High Pressure Liquid
Excipients
General pharmacology
Hydrogen-Ion Concentration
Indicators and Reagents
Medical sciences
Pharmaceutical formulations
Pharmacology. Drug treatments
Reference Standards
Reversed-phase ion-pair high-performance liquid chromatography
Solubility
Tablets, Enteric-Coated
Vitamin B Complex - analysis
Vitamins - analysis
Water-soluble vitamins
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Summary:A reversed-phase ion-pair high-performance liquid chromatografic method (RP-IPC) was developed to assay some water-soluble vitamins in solution dosage forms. Vitamins of the B-group B 1, B 2, B 3, and B 6, including vitamin C were determined in Oligovit ® coated tablets. In Beviplex ® coated tablets the vitamins B 1, B 2, B 3, B 6 and p-aminobenzoic acid were analysed. Hexanesulphonic acid sodium salt and triethanolamine in water–methanol were used as mobile phase with adjusting pH to 2.8 with orthophosphoric acid. Phenol was used as an internal standard. For quantitative simultaneous analysis of vitamins in pharmaceutical formulations, the method of internal standard was used. All parameters for the validation of the method are given.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(98)00109-5