Biochemical and Electron Microscopic Image Analysis of the Hexameric E1 Helicase

• Published in: The Journal of biological chemistry 1999-02, Vol.274 (7), p.4447-4458
• Main Authors: Fouts, E T, Yu, X, Egelman, E H, Botchan, M R
• Format: Article
• Language: English
• Subjects: Animals; Bovine papillomavirus 1 - enzymology; Bovine papillomavirus 1 - physiology; Cell Line; DNA Helicases - chemistry; DNA Helicases - metabolism; DNA Helicases - ultrastructure; DNA Replication; DNA, Viral - metabolism; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - metabolism; DNA-Binding Proteins - ultrastructure; Enhancer Elements, Genetic; Microscopy, Electron; Models, Molecular; Nucleic Acid Conformation; Papillomavirus; Protein Conformation; Protein Folding; Spodoptera; Viral Proteins - chemistry; Viral Proteins - metabolism; Viral Proteins - ultrastructure; Virus Replication
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.274.7.4447

DNA replication initiator proteins bind site specifically to origin sites and in most cases participate in the early steps of unwinding the duplex. The papillomavirus preinitiation complex that assembles on the origin of replication is composed of proteins E1 and the activator protein E2. E2 is an ancillary factor that increases the affinity of E1 for the ori site through cooperative binding. Here we show that duplex DNA affects E1 (in the absence of E2) to assemble into an active hexameric structure. As a 10-base oligonucleotide can also induce this oligomerization, it seems likely that DNA binding allosterically induces a conformation that enhances hexamers. E1 assembles as a bi-lobed, presumably double hexameric structure on duplex DNA and can initiate bi-directional unwinding from an ori site. The DNA takes an apparent straight path through the double hexamers. Image analysis of E1 hexameric rings shows that the structures are heterogeneous and have either a 6- or 3-fold symmetry. The rings are about 40–50 Å thick and 125 Å in diameter. The density of the central cavity appears to be a variable and we speculate that a plugged center may represent a conformational flexibility of a subdomain of the monomer, to date unreported for other hexameric helicases.