Critical islet mass for successful porcine islet autotransplantation

A major reason for the failure of clinical islet transplantations may be a limited islet mass. The aim of this study was to determine the critical islet mass necessary for normalization of glucose metabolism in a porcine model. Diabetes was induced by total pancreatectomy. The splenic lobe of the pa...

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Bibliographic Details
Published in:Journal of molecular medicine (Berlin, Germany) Germany), 1999-01, Vol.77 (1), p.126-129
Main Authors: Mellert, J, Hering, B J, Liu, X, Brandhorst, D, Brandhorst, H, Pfeffer, F, Federlin, K, Bretzel, R G, Hopt, U T
Format: Article
Language:English
Subjects:
Animals
Blood Glucose - metabolism
Diabetes Mellitus, Type 1 - metabolism
Diabetes Mellitus, Type 1 - surgery
Female
Glucose Tolerance Test
Graft Survival
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - cytology
Islets of Langerhans - metabolism
Islets of Langerhans Transplantation
Pancreatectomy
Swine
Swine, Miniature
Transplantation, Autologous
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Summary:A major reason for the failure of clinical islet transplantations may be a limited islet mass. The aim of this study was to determine the critical islet mass necessary for normalization of glucose metabolism in a porcine model. Diabetes was induced by total pancreatectomy. The splenic lobe of the pancreas was intraductally distended with UW-solution containing 2.67-3.33 mg/ml collagenase, and the distended pancreas was digested in a continuous digestion filtration device. The islets were purified on a isoosmotic Ficoll-sodium-diatrizoate gradient. The survival period of the diabetic recipients in group 2 and 3 receiving, respectively, a low (2.14+/-0.39 microL/kg body weight) and a high (4.99+/-0.83 microL/kg body weight) islet mass was significantly prolonged compared to that of diabetic recipients in group 1 receiving no islet transplantation. However, the survival period of the recipients in group 2 was not significantly different to that in group 3. Three recipients of an islet mass of >5 microl/kg body weight became normoglycemic (fasting blood glucose
ISSN:0946-2716
DOI:10.1007/s001090050319