LC/MS/MS analysis of vesnarinone and its principal metabolites in plasma and urine

An LC/MS/MS assay was developed and successfully used to quantitate vesnarinone and its principal metabolites (OPC-8230, OPC-18136, and OPC-18137) in human plasma and urine. Samples were pre-treated with liquid–solid extraction followed by simultaneous monitoring of primary and daughter ions which w...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1999, Vol.18 (6), p.1037-1045
Main Authors: Tata, PrasadN.V., Schorno, KarlS, Cowart, T.Douglas, Bramer, StevenL
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Biotransformation
Cardiotonic Agents - analysis
Cardiotonic Agents - blood
Cardiotonic Agents - urine
Chromatography, Liquid
General pharmacology
Humans
LC/MS/MS
Mass Spectrometry
Medical sciences
Metabolites
Parent ion
Pharmacology. Drug treatments
Product ion
Quinolines - analysis
Quinolines - blood
Quinolines - urine
Reproducibility of Results
Spectrophotometry, Ultraviolet
Vesnarinone
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Summary:An LC/MS/MS assay was developed and successfully used to quantitate vesnarinone and its principal metabolites (OPC-8230, OPC-18136, and OPC-18137) in human plasma and urine. Samples were pre-treated with liquid–solid extraction followed by simultaneous monitoring of primary and daughter ions which were used for the identification and quantitation of the analytes on LC/MS/MS. This assay offers advantages of specificity, speed and greater sensitivity over the previously developed HPLC-UV assay. The lower limit of quantitation is 500 ng ml −1 for vesnarinone and 20 ng ml −1 for OPC-8230, OPC-18137, and OPC-18136 in plasma. Methodology is similar for the estimation of these analytes in urine with the lower limit of quantitation being 500 ng ml −1 for vesnarinone and 100 ng ml −1 for each metabolite. Ascorbic acid was added to stabilize the analytes from degradation. This LC/MS/MS method was developed to overcome many practical problems associated with the HPLC method. The LC/MS/MS method offers the flexibility of analyzing additional metabolites and changing the linearity range to accommodate the differences in linear range (200–10 000 ng ml −1 for vesnarinone and 20–1000 for metabolites) for the analytes.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(98)00194-0