A routine high-performance size-exclusion chromatography to determine molecular size distribution of Haemophilus influenzae type b conjugate vaccines

High-performance size exclusion chromatography has been used to determine the molecular size distribution of Haemophilus influenzae type b (Hib) conjugate vaccines. Both high molecular weight preparations of native Hib capsular polysaccharide coupled to tetanus toxoid and low molecular weight vaccin...

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Bibliographic Details
Published in:Vaccine 1999, Vol.17 (2), p.118-125
Main Authors: von Hunolstein, C, Parisi, L, Recchia, S
Format: Article
Language:English
Subjects:
Bacterial Proteins - administration & dosage
Bacteriology
Biological and medical sciences
Chromatography, Gel - methods
Chromatography, Gel - statistics & numerical data
conjugate vaccines
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
Haemophilus influenzae
Haemophilus influenzae type b
Haemophilus influenzae type b - immunology
Haemophilus Vaccines - administration & dosage
Haemophilus Vaccines - chemistry
HPSEC
Humans
Microbiology
Molecular Weight
Sensitivity and Specificity
Tetanus Toxoid - administration & dosage
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Conjugate - administration & dosage
Vaccines, Conjugate - chemistry
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Summary:High-performance size exclusion chromatography has been used to determine the molecular size distribution of Haemophilus influenzae type b (Hib) conjugate vaccines. Both high molecular weight preparations of native Hib capsular polysaccharide coupled to tetanus toxoid and low molecular weight vaccines with Hib oligosaccharides linked to the CRM 197 nontoxic mutant diphtheria protein were analysed. Columns with different fractionation ranges were used for the two kinds of vaccines. This method showed to be rapid, accurate and reproducible for different lots of Hib vaccine of different composition produced by various manufacturers. It could replace more time-consuming chromatographic methods enabling control authorities to employ a single methodological approach for different Hib vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(98)00166-2