HIV-1 gp160 decreases the K + voltage-gated current from Jurkat E6.1 T cells by up-phosphorylation

HIV-1 gp120/gp160 is known to disturb the activity of p56 lck, protein kinase C (PKC) and Ca 2+ homeostasis in T lymphocytes. We found that gp160 decreases the Kv1.3 current of Jurkat E6.1 cells probably by increasing the PKC-dependent phosphorylation of Kv channel protein after 5 days. This decreas...

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Bibliographic Details
Published in:FEBS letters 1999-01, Vol.443 (2), p.187-191
Main Authors: Dellis, Olivier, Bouteau, François, Guenounou, Moncef, Rona, Jean-Pierre
Format: Article
Language:English
Subjects:
4-AP, 4-aminopyridine
AIDS/HIV
E m, membrane potential
HIV Envelope Protein gp160 - metabolism
HIV Envelope Protein gp160 - physiology
HIV-1 gp160
HIV-1, human immunodeficiency virus type 1
Human T cell
Humans
Ion Channel Gating
Jurkat Cells
Kv1.3 Potassium Channel
Membrane potential
OA, okadaic acid
p56 lck
p56lck
Phosphorylation
PKC, protein kinase C
Potassium channel
Potassium Channel Blockers
Potassium Channels
Potassium Channels, Voltage-Gated
Protein kinase C
Stauro, staurosporine
TPA, 12-O-tetradecanoyl phorbol 13-acetate
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Summary:HIV-1 gp120/gp160 is known to disturb the activity of p56 lck, protein kinase C (PKC) and Ca 2+ homeostasis in T lymphocytes. We found that gp160 decreases the Kv1.3 current of Jurkat E6.1 cells probably by increasing the PKC-dependent phosphorylation of Kv channel protein after 5 days. This decrease is dose-dependent. In contrast, gp160 did not decrease the Kv1.3 current of the JCaM1.6 cell line, a p56 lck-defective Jurkat cell line. This shows that p56 lck was at the beginning of the events which induced the Kv1.3 current decrease. As a consequence of this decrease, Jurkat E6.1 cells were depolarized and exhibited a volume increase.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)01691-3