Differing Roles of Adenosine Receptor Subtypes in Retinal Ischemia-Reperfusion Injury in the Rat

Adenosine has been shown to be a major component of the retina's endogenous reaction to ischemia. In earlier studies, the significant changes in adenosine concentration that occur during ischemia and the ensuing reperfusion period were documented. While previous studies have shown that adenosin...

Full description

Saved in:
Bibliographic Details
Published in:Experimental eye research 1999-01, Vol.68 (1), p.9-17
Main Authors: LI, BING, ROSENBAUM, PEARL S., JENNINGS, NUALA M., MAXWELL, KELLY M., ROTH, STEVEN
Format: Article
Language:English
Subjects:
adenosine
Animals
Biological and medical sciences
Caffeine - analogs & derivatives
Caffeine - pharmacology
CSC
DPCPX
Electroretinography
ischemia
Medical sciences
Ophthalmology
Purinergic P1 Receptor Antagonists
rat
Rats
Rats, Sprague-Dawley
Receptors, Purinergic P1 - physiology
Reperfusion Injury - physiopathology
retina
Retina - drug effects
Retina - physiology
Retinopathies
Time Factors
Xanthines - pharmacology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Adenosine has been shown to be a major component of the retina's endogenous reaction to ischemia. In earlier studies, the significant changes in adenosine concentration that occur during ischemia and the ensuing reperfusion period were documented. While previous studies have shown that adenosine is a mediator of the changes in blood flow that occur in response to ischemia, hypoxia, and hypoglycemia in the retina, little is known about other functional effects that result from these changes in adenosine concentration. Accordingly, the influence of adenosine receptor blockade on the functional and histological outcome following ischemia in rats was examined. Specific antagonists of the adenosine A1 and A2a receptors were injected systemically, prior to ischemia of either 5, 30, or 60 min. The recovery of the electroretinogram a and b waves was followed for up to 7 days after ischemia, and retinal structure was examined by light microscopy. The adenosine A1 receptor antagonist DPCPX attenuated recovery after retinal ischemia of either 5 or 30 min, while the A2a receptor antagonist CSC dramatically protected retinal function and structure even with ischemia lasting up to 60 min. It was concluded that blockade of the A2a receptor, possibly combined with stimulation of the A1 receptor, may represent a potential new strategy for the prevention of ischemic damage in the retina.
ISSN:0014-4835
1096-0007
DOI:10.1006/exer.1998.0573