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Promoter-directed expression of recombinant fire-fly luciferase in the salivary glands of Hermes-transformed Aedes aegypti
Molecular genetic analyses of biological properties characteristic of insect vectors of disease, such as hematophagy and competence for pathogens, require the ability to isolate and characterize genes involved in these processes. We have been working to develop molecular approaches for studying the...
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| Published in: | Gene 1999-01, Vol.226 (2), p.317-325 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c481t-2576a3bd5bc59f7467098507878aac703a015e14b89e485fefa2ea816bd8627d3 |
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| cites | cdi_FETCH-LOGICAL-c481t-2576a3bd5bc59f7467098507878aac703a015e14b89e485fefa2ea816bd8627d3 |
| container_end_page | 325 |
| container_issue | 2 |
| container_start_page | 317 |
| container_title | Gene |
| container_volume | 226 |
| creator | Coates, Craig J Jasinskiene, Nijole Pott, Gregory B James, Anthony A |
| description | Molecular genetic analyses of biological properties characteristic of insect vectors of disease, such as hematophagy and competence for pathogens, require the ability to isolate and characterize genes involved in these processes. We have been working to develop molecular approaches for studying the promoter function of genes that are expressed specifically in the adult salivary glands of the yellow fever mosquito,
Aedes aegypti. Genomic DNA fragments containing
cis-acting promoter elements from the
Maltase-like I (
MalI) and
Apyrase (
Apy) genes were cloned so as to direct the expression of the reporter gene,
luciferase (
luc). The function of the promoters was assayed transiently in cultured insect cells and by germ-line transformation of
Ae. aegypti.
MalI and
Apy DNA fragments consisting of at least 650 nucleotides (nt) of DNA immediately adjacent to the 5′-end of the initiation codon of the mosquito genes directed constitutive expression of the
luc reporter gene in cultured cells. When introduced into
Ae. aegypti chromosomes, approximately 1.5 kilobases (kb) of each promoter were able to direct the predicted developmental-, sex- and tissue-specific expression of the reporter gene in patterns identical to those determined for the respective endogenous genes. |
| doi_str_mv | 10.1016/S0378-1119(98)00557-5 |
| format | article |
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Aedes aegypti. Genomic DNA fragments containing
cis-acting promoter elements from the
Maltase-like I (
MalI) and
Apyrase (
Apy) genes were cloned so as to direct the expression of the reporter gene,
luciferase (
luc). The function of the promoters was assayed transiently in cultured insect cells and by germ-line transformation of
Ae. aegypti.
MalI and
Apy DNA fragments consisting of at least 650 nucleotides (nt) of DNA immediately adjacent to the 5′-end of the initiation codon of the mosquito genes directed constitutive expression of the
luc reporter gene in cultured cells. When introduced into
Ae. aegypti chromosomes, approximately 1.5 kilobases (kb) of each promoter were able to direct the predicted developmental-, sex- and tissue-specific expression of the reporter gene in patterns identical to those determined for the respective endogenous genes.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(98)00557-5</identifier><identifier>PMID: 9931506</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aedes - cytology ; Aedes - enzymology ; Aedes - genetics ; Aedes aegypti ; Aedes albopictus ; Animals ; Apy gene ; apy promoter ; Apyrase gene ; Base Sequence ; bioluminescence ; Blotting, Northern ; Cell Line ; Coleoptera - enzymology ; Culicidae ; cultured cells ; Disease vector ; DNA Primers ; Drosophila melanogaster ; enzyme activity ; Female ; gene expression ; gene transfer ; Genes, Reporter ; Genetic transformation ; histochemistry ; luciferase ; Luciferases - genetics ; mal1 promoter ; MalI gene ; Maltase-like I gene ; messenger RNA ; Mosquito ; plasmid vectors ; Promoter analysis ; promoter regions ; Promoter Regions, Genetic ; recombinant DNA ; Recombinant Proteins - genetics ; reporter genes ; RNA, Messenger - genetics ; salivary glands ; Salivary Glands - enzymology ; Transformation, Genetic ; transgenic animals</subject><ispartof>Gene, 1999-01, Vol.226 (2), p.317-325</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-2576a3bd5bc59f7467098507878aac703a015e14b89e485fefa2ea816bd8627d3</citedby><cites>FETCH-LOGICAL-c481t-2576a3bd5bc59f7467098507878aac703a015e14b89e485fefa2ea816bd8627d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9931506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coates, Craig J</creatorcontrib><creatorcontrib>Jasinskiene, Nijole</creatorcontrib><creatorcontrib>Pott, Gregory B</creatorcontrib><creatorcontrib>James, Anthony A</creatorcontrib><title>Promoter-directed expression of recombinant fire-fly luciferase in the salivary glands of Hermes-transformed Aedes aegypti</title><title>Gene</title><addtitle>Gene</addtitle><description>Molecular genetic analyses of biological properties characteristic of insect vectors of disease, such as hematophagy and competence for pathogens, require the ability to isolate and characterize genes involved in these processes. We have been working to develop molecular approaches for studying the promoter function of genes that are expressed specifically in the adult salivary glands of the yellow fever mosquito,
Aedes aegypti. Genomic DNA fragments containing
cis-acting promoter elements from the
Maltase-like I (
MalI) and
Apyrase (
Apy) genes were cloned so as to direct the expression of the reporter gene,
luciferase (
luc). The function of the promoters was assayed transiently in cultured insect cells and by germ-line transformation of
Ae. aegypti.
MalI and
Apy DNA fragments consisting of at least 650 nucleotides (nt) of DNA immediately adjacent to the 5′-end of the initiation codon of the mosquito genes directed constitutive expression of the
luc reporter gene in cultured cells. When introduced into
Ae. aegypti chromosomes, approximately 1.5 kilobases (kb) of each promoter were able to direct the predicted developmental-, sex- and tissue-specific expression of the reporter gene in patterns identical to those determined for the respective endogenous genes.</description><subject>Aedes - cytology</subject><subject>Aedes - enzymology</subject><subject>Aedes - genetics</subject><subject>Aedes aegypti</subject><subject>Aedes albopictus</subject><subject>Animals</subject><subject>Apy gene</subject><subject>apy promoter</subject><subject>Apyrase gene</subject><subject>Base Sequence</subject><subject>bioluminescence</subject><subject>Blotting, Northern</subject><subject>Cell Line</subject><subject>Coleoptera - enzymology</subject><subject>Culicidae</subject><subject>cultured cells</subject><subject>Disease vector</subject><subject>DNA Primers</subject><subject>Drosophila melanogaster</subject><subject>enzyme activity</subject><subject>Female</subject><subject>gene expression</subject><subject>gene transfer</subject><subject>Genes, Reporter</subject><subject>Genetic transformation</subject><subject>histochemistry</subject><subject>luciferase</subject><subject>Luciferases - genetics</subject><subject>mal1 promoter</subject><subject>MalI gene</subject><subject>Maltase-like I gene</subject><subject>messenger RNA</subject><subject>Mosquito</subject><subject>plasmid vectors</subject><subject>Promoter analysis</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant DNA</subject><subject>Recombinant Proteins - genetics</subject><subject>reporter genes</subject><subject>RNA, Messenger - genetics</subject><subject>salivary glands</subject><subject>Salivary Glands - enzymology</subject><subject>Transformation, Genetic</subject><subject>transgenic animals</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxS0EKsvCR6jwCcHBYMfxvxOqKqBIlUAqPVuOM16Mknixs1WXT4_TrHrtyZbnN34z7yF0zuhHRpn8dEO50oQxZt4b_YFSIRQRz9CGaWUIpVw_R5tH5CV6VcofShesOUNnxnAmqNygfz9zGtMMmfQxg5-hx3C_z1BKTBNOAdfHNHZxctOMQ0VIGI54OPgYILsCOE54_g24uCHeuXzEu8FNfVk6ryCPUMic3VRCqvceX0APBTvYHfdzfI1eBDcUeHM6t-j265dfl1fk-se375cX18S3ms2kEUo63vWi88IE1UpFjRZUaaWd84pyR5kA1nbaQKtFgOAacJrJrteyUT3fonfrv_uc_h6gzHaMxcNQB4V0KFaaqsCleBJkmjeMVuu2SKygz6mUDMHucxzr9pZRu4RjH8Kxi_PWaPsQjl0Ezk8Ch67a8dh1SqPW36714JJ1uxyLvb1pKOO00YZL2Vbi80pANewuQrbFR5g8rOnZPsUnZvgPjhmprQ</recordid><startdate>19990121</startdate><enddate>19990121</enddate><creator>Coates, Craig J</creator><creator>Jasinskiene, Nijole</creator><creator>Pott, Gregory B</creator><creator>James, Anthony A</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990121</creationdate><title>Promoter-directed expression of recombinant fire-fly luciferase in the salivary glands of Hermes-transformed Aedes aegypti</title><author>Coates, Craig J ; Jasinskiene, Nijole ; Pott, Gregory B ; James, Anthony A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-2576a3bd5bc59f7467098507878aac703a015e14b89e485fefa2ea816bd8627d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Aedes - cytology</topic><topic>Aedes - enzymology</topic><topic>Aedes - genetics</topic><topic>Aedes aegypti</topic><topic>Aedes albopictus</topic><topic>Animals</topic><topic>Apy gene</topic><topic>apy promoter</topic><topic>Apyrase gene</topic><topic>Base Sequence</topic><topic>bioluminescence</topic><topic>Blotting, Northern</topic><topic>Cell Line</topic><topic>Coleoptera - enzymology</topic><topic>Culicidae</topic><topic>cultured cells</topic><topic>Disease vector</topic><topic>DNA Primers</topic><topic>Drosophila melanogaster</topic><topic>enzyme activity</topic><topic>Female</topic><topic>gene expression</topic><topic>gene transfer</topic><topic>Genes, Reporter</topic><topic>Genetic transformation</topic><topic>histochemistry</topic><topic>luciferase</topic><topic>Luciferases - genetics</topic><topic>mal1 promoter</topic><topic>MalI gene</topic><topic>Maltase-like I gene</topic><topic>messenger RNA</topic><topic>Mosquito</topic><topic>plasmid vectors</topic><topic>Promoter analysis</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant DNA</topic><topic>Recombinant Proteins - genetics</topic><topic>reporter genes</topic><topic>RNA, Messenger - genetics</topic><topic>salivary glands</topic><topic>Salivary Glands - enzymology</topic><topic>Transformation, Genetic</topic><topic>transgenic animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coates, Craig J</creatorcontrib><creatorcontrib>Jasinskiene, Nijole</creatorcontrib><creatorcontrib>Pott, Gregory B</creatorcontrib><creatorcontrib>James, Anthony A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coates, Craig J</au><au>Jasinskiene, Nijole</au><au>Pott, Gregory B</au><au>James, Anthony A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promoter-directed expression of recombinant fire-fly luciferase in the salivary glands of Hermes-transformed Aedes aegypti</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1999-01-21</date><risdate>1999</risdate><volume>226</volume><issue>2</issue><spage>317</spage><epage>325</epage><pages>317-325</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Molecular genetic analyses of biological properties characteristic of insect vectors of disease, such as hematophagy and competence for pathogens, require the ability to isolate and characterize genes involved in these processes. We have been working to develop molecular approaches for studying the promoter function of genes that are expressed specifically in the adult salivary glands of the yellow fever mosquito,
Aedes aegypti. Genomic DNA fragments containing
cis-acting promoter elements from the
Maltase-like I (
MalI) and
Apyrase (
Apy) genes were cloned so as to direct the expression of the reporter gene,
luciferase (
luc). The function of the promoters was assayed transiently in cultured insect cells and by germ-line transformation of
Ae. aegypti.
MalI and
Apy DNA fragments consisting of at least 650 nucleotides (nt) of DNA immediately adjacent to the 5′-end of the initiation codon of the mosquito genes directed constitutive expression of the
luc reporter gene in cultured cells. When introduced into
Ae. aegypti chromosomes, approximately 1.5 kilobases (kb) of each promoter were able to direct the predicted developmental-, sex- and tissue-specific expression of the reporter gene in patterns identical to those determined for the respective endogenous genes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9931506</pmid><doi>10.1016/S0378-1119(98)00557-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1999-01, Vol.226 (2), p.317-325 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | Elsevier |
| subjects | Aedes - cytology Aedes - enzymology Aedes - genetics Aedes aegypti Aedes albopictus Animals Apy gene apy promoter Apyrase gene Base Sequence bioluminescence Blotting, Northern Cell Line Coleoptera - enzymology Culicidae cultured cells Disease vector DNA Primers Drosophila melanogaster enzyme activity Female gene expression gene transfer Genes, Reporter Genetic transformation histochemistry luciferase Luciferases - genetics mal1 promoter MalI gene Maltase-like I gene messenger RNA Mosquito plasmid vectors Promoter analysis promoter regions Promoter Regions, Genetic recombinant DNA Recombinant Proteins - genetics reporter genes RNA, Messenger - genetics salivary glands Salivary Glands - enzymology Transformation, Genetic transgenic animals |
| title | Promoter-directed expression of recombinant fire-fly luciferase in the salivary glands of Hermes-transformed Aedes aegypti |
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