Role of electrostatics on membrane binding, aggregation and destabilization induced by NAD(P)H dehydrogenases. Implication in membrane fusion

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered a classical glycolytic protein that can promote the fusion of phospholipid vesicles and can also play a vital role on in vivo fusogenic events. However, it is not clear how this redox enzyme, which lack conserved structural or sequence m...

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Bibliographic Details
Published in:Biophysical chemistry 2008-10, Vol.137 (2), p.126-132
Main Authors: Avila, César L., de Arcuri, Beatriz F., Gonzalez-Nilo, Fernando, De Las Rivas, Javier, Chehín, Rosana, Morero, Roberto
Format: Article
Language:English
Subjects:
Alcohol Dehydrogenase - chemistry
Animals
Base Sequence
Cattle
Conserved Sequence
FDPB
Glutamate Dehydrogenase (NADP+) - chemistry
Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) - chemistry
L-Iditol 2-Dehydrogenase - chemistry
Membrane Fusion
Models, Molecular
NADH, NADPH Oxidoreductases - chemistry
Phosphatidylcholines - chemistry
Phosphatidylserines - chemistry
Protein Structure, Secondary
Protein–membrane interactions
Rabbits
Sheep
Spectrometry, Fluorescence
Static Electricity
Thermodynamics
Unilamellar Liposomes - chemistry
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Summary:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered a classical glycolytic protein that can promote the fusion of phospholipid vesicles and can also play a vital role on in vivo fusogenic events. However, it is not clear how this redox enzyme, which lack conserved structural or sequence motifs related to membrane fusion, catalyze this process. In order to detect if this ability is present in other NAD(P)H dehydrogenases with available structure, spectroscopic studies were performed to evaluate the capability of alcohol dehydrogenase (ADH), glutamic dehydrogenase (GDH) and sorbitol dehydrogenase (SDH) to bind, aggregate, destabilize and fuse vesicles. Based on finite difference Poisson–Boltzmann calculations (FDPB) the protein–membrane interactions were analyzed. A model for the protein–membrane complex in its minimum free energy of interaction was obtained for each protein and the amino acids involved in the binding processes were suggested. A previously undescribed relationship between membrane destabilization and crevices with high electropositive potential on the protein surface was proposed. The putative implication of the non-specific electrostatics on NAD(P)H dehydrogenases induced membrane fusion is discussed.
ISSN:0301-4622
1873-4200
DOI:10.1016/j.bpc.2008.08.003