Sequence scanning chicken cosmids: a methodology for genome screening

The chicken genome is relatively poorly studied at the molecular level. The karyotype 2 n=78 is divided into three main chromosomal sub-groups: the macrochromosomes (six pairs), the intermediate microchromosomes (four pairs) and the microchromosomes (29 pairs). Whilst the microchromosome group compr...

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Bibliographic Details
Published in:Gene 1999-02, Vol.227 (2), p.223-230
Main Authors: Clark, M.S., Edwards, Y.J.K., McQueen, H.A., Meek, S.E., Smith, S., Umrania, Y., Warner, S., Williams, G., Elgar, G.
Format: Article
Language:English
Subjects:
Animals
biochemical techniques
Chicken
chickens
Chickens - genetics
Chromosome Mapping
chromosomes
Chromosomes - genetics
Chromosomes, Human, Pair 12 - genetics
Cloning, Molecular
Cosmid scanning
cosmids
Cosmids - genetics
Databases, Factual
Gene mapping
genes
Genetic Linkage - genetics
Genome
genomics
Humans
Macrochromosome
Microchromosome
microchromosomes
Microsatellite Repeats - genetics
Molecular Sequence Data
Sequence Analysis, DNA
sequence scanning technique
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Summary:The chicken genome is relatively poorly studied at the molecular level. The karyotype 2 n=78 is divided into three main chromosomal sub-groups: the macrochromosomes (six pairs), the intermediate microchromosomes (four pairs) and the microchromosomes (29 pairs). Whilst the microchromosome group comprise only 25% of the DNA, increasing evidence is proving that this is disproportionate to their gene content. This paper demonstrates the utility of cosmid sequence scanning as a potential method for analysing the chicken genome, providing an economical method for the production of a molecular map. The GC content, gene density and repeat distribution are analysed relative to chromosomal origin. Results indicate that gene density is higher on the microchromosomes. During the scanning process an example of conserved linkage between chicken and human (12q34.2) has been demonstrated.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(98)00610-6