Cloning and characterization of pl1 encoding an in planta-secreted pectate lyase of Fusarium oxysporum

A pectate lyase (PL1) from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici was previously characterized, and evidence was obtained for its production in planta. The gene encoding PL1 was isolated from a genomic library of F. oxysporum f. sp. lycopersici. Pl1 encodes a 240 amin...

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Bibliographic Details
Published in:Current genetics 1999-02, Vol.35 (1), p.36-40
Main Authors: Huertas-Gonzalez, M.D, Ruiz-Roldan, M.C, Garcia Maceira, F.I, Roncero, M.I.G, Pietro, A. di
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
cloning
Cloning, Molecular
fungal diseases of plants
Fungal Proteins - genetics
Fusarium - enzymology
Fusarium - pathogenicity
Fusarium oxysporum
Fusarium oxysporum f. sp. lycopersici
genbank/af080485
gene expression
Gene Expression Regulation, Fungal
Genes, Fungal - genetics
introns
Lycopersicon esculentum - microbiology
messenger RNA
Molecular Sequence Data
nucleotide sequences
pectate lyase
pectins
Plant Diseases - microbiology
polygalacturonic acid
Polysaccharide-Lyases - genetics
RNA, Messenger - metabolism
roots
Sequence Analysis, DNA
Sequence Homology, Amino Acid
signal peptide
Solanum lycopersicum var. lycopersicum
stems
structural genes
tomatoes
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Summary:A pectate lyase (PL1) from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici was previously characterized, and evidence was obtained for its production in planta. The gene encoding PL1 was isolated from a genomic library of F. oxysporum f. sp. lycopersici. Pl1 encodes a 240 amino-acid polypeptide with one putative N-glycosylation site and a 15 amino-acid N-terminal signal peptide. PL1 showed 89%, 67%, 55% and 56% identity with the products of the Fusarium solani f.sp. pisi pelA, pelB, pelC and pelD genes, respectively. A single copy of the gene was detected in different formae speciales of F. oxysporum. The pl1 transcript was observed during growth on polygalacturonic acid sodium salt and tomato vascular tissue, but not on pectin or glucose. RT-PCR showed pl1 expression in roots and stems of tomato plants infected by F. oxysporum f.sp. lycopersici.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050430