Bacterial Entry to the Splenic White Pulp Initiates Antigen Presentation to CD8 + T Cells

The spleen plays an important role in host-protective responses to bacteria. However, the cellular dynamics that lead to pathogen-specific immunity remain poorly understood. Here we examined Listeria monocytogenes (Lm) infection in the mouse spleen via in situ fluorescence microscopy. We found that...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 2008-09, Vol.29 (3), p.476-486
Main Authors: Aoshi, Taiki, Zinselmeyer, Bernd H., Konjufca, Vjollca, Lynch, Jennifer N., Zhang, Xin, Koide, Yukio, Miller, Mark J.
Format: Article
Language:English
Subjects:
Animals
Antigen presentation
Antigen Presentation - drug effects
Bacteria
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - drug effects
CD8-Positive T-Lymphocytes - immunology
CELLIMMUNO
Clodronic Acid - pharmacology
Dendritic Cells - drug effects
Dendritic Cells - immunology
Dendritic Cells - metabolism
Experiments
Immune system
Infections
Liposomes
Listeria monocytogenes - immunology
Listeriosis - immunology
Listeriosis - microbiology
Mice
Mice, Inbred BALB C
Mice, Transgenic
Molecular weight
Motility
Permeability
Pertussis Toxin - pharmacology
Rodents
Software
Spleen - cytology
Spleen - drug effects
Spleen - immunology
Spleen - microbiology
Studies
Whooping cough
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Summary:The spleen plays an important role in host-protective responses to bacteria. However, the cellular dynamics that lead to pathogen-specific immunity remain poorly understood. Here we examined Listeria monocytogenes (Lm) infection in the mouse spleen via in situ fluorescence microscopy. We found that the redistribution of Lm from the marginal zone (MZ) to the periarteriolar lymphoid sheath (PALS) was inhibited by pertussis toxin and required the presence of CD11c + cells. As early as 9 hr after infection, we detected infected dendritic cells in the peripheral regions of the PALS and clustering of Lm-specific T cells by two-photon microscopy. Pertussis toxin inhibited both Lm entry into the PALS and antigen presentation to CD8 + T cells. Our study suggests that splenic dendritic cells rapidly deliver intracellular bacteria to the T cell areas of the white pulp to initiate CD8 + T cell responses.
ISSN:1074-7613
1097-4180
DOI:10.1016/j.immuni.2008.06.013