hBolA, novel non-classical secreted proteins, belonging to different BolA family with functional divergence

This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected C...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry 2008-10, Vol.317 (1-2), p.61-68
Main Authors: Zhou, Yu-Bo, Cao, Jia-Bing, Wan, Bing-Bing, Wang, Xin-Rong, Ding, Guo-Hui, Zhu, Hong, Yang, Hong-Meng, Wang, Ke-Sheng, Zhang, Xin, Han, Ze-Guang
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biochemistry
Bioinformatics
Biomedical and Life Sciences
Cardiology
Cercopithecus aethiops
Computational Biology
COS Cells
Eukaryotic Cells - metabolism
Evolution, Molecular
Genetic Variation
Humans
Life Sciences
Medical Biochemistry
Mitochondrial Proteins
Molecular biology
Molecular Sequence Data
Mutant Proteins - secretion
Oncology
Peptides
Phylogeny
Protein Sorting Signals
Protein Transport
Proteins
Proteins - chemistry
Proteins - secretion
Recombinant Fusion Proteins - secretion
Subcellular Fractions - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Furthermore, the secretion was not dependent on its predicted signal peptide. All the experiment results suggested that the secretion was a non-classical export. Phylogenetic analysis showed that the human BolAs belong to three different groups with functional divergence of BolA subfamily, where the different helix-turn-helix motif among hBolA1, hBolA2, and hBolA3 could be responsible for their functional divergence. Our data provided a basis for functional studies of BolA protein family.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-008-9809-2