Use of anti-glycoprotein monoclonal antibodies to characterize rabies virus in formalin-fixed tissues

Seventy anti-rabies virus monoclonal antibodies (Mabs) were tested for reactivity with rabies and rabies-related viruses in formalin-fixed (FF) tissues. Forty-three of the Mabs were directed against the glycoprotein and 27 were directed against the nucleocapsid as determined by enzyme immunoassays a...

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Bibliographic Details
Published in:Journal of virological methods 1999, Vol.77 (1), p.69-74
Main Authors: Warner, Cynthia, Fekadu, Makonnen, Whitfield, Sylvia, Shaddock, John
Format: Article
Language:English
Subjects:
Animals
Anti-glycoprotein monoclonal antibodies
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antigens, Viral - analysis
Australian bat virus
Biological and medical sciences
Brain - virology
Formaldehyde
Formalin-fixed detection
Fundamental and applied biological sciences. Psychology
Immunoenzyme Techniques
Lyssavirus
Mice
Microbiology
Neutralization Tests
Rabies
Rabies - virology
Rabies virus
Rabies virus - immunology
Rabies virus - isolation & purification
Rabies-related virus
Techniques used in virology
Tissue Fixation
Viral Envelope Proteins - immunology
Virology
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Summary:Seventy anti-rabies virus monoclonal antibodies (Mabs) were tested for reactivity with rabies and rabies-related viruses in formalin-fixed (FF) tissues. Forty-three of the Mabs were directed against the glycoprotein and 27 were directed against the nucleocapsid as determined by enzyme immunoassays and neutralization tests. Twenty of the anti-glycoprotein Mabs and one of the anti-nucleocapsid Mabs reacted with the rabies challenge virus strain (CVS) in FF tissue. These 21 Mabs were screened against other lyssaviruses in FF tissues: five rabies virus strains (coyote, skunk, raccoon, red bat, and silver-haired bat), and four rabies-related viruses (Australian bat lyssavirus, Duvenhage virus, Lagos bat virus, and Mokola virus). One of the anti-glycoprotein Mabs was reactive with all the virus strains screened. Another of the anti-glycoprotein Mabs reacted with all of the rabies virus strains tested, but not with any of the rabies-related virus strains tested. The remaining Mabs had reactivity patterns that could be useful for characterizing lyssaviruses in FF tissues.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(98)00136-0