Microencapsulated human hair dermal papilla cells: a substitute for dermal papilla?

Dermal papillae (DP) play a pivotal role in hair formation, growth and cycling. However, the number of DP is limited. In this study, we report the production of “reconstructed DP” by enclosing DP cells within an alginate–polylysine–alginate (APA) semipermeable membrane. MTT assay and electron micros...

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Bibliographic Details
Published in:Archives of Dermatological Research 2008-10, Vol.300 (9), p.531-535
Main Authors: Lin, Chang-min, Li, Yu, Ji, Ying-chang, Keng, Huang, Cai, Xiang-na, Zhang, Jin-kun
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Dermatology
Dermis - cytology
Dermis - physiology
Diffusion Chambers, Culture - methods
Drug Compounding - methods
Epithelial Cells - cytology
Epithelial Cells - physiology
Female
Hair Follicle - cytology
Hair Follicle - physiology
Humans
Male
Medical sciences
Medicine
Medicine & Public Health
Rats
Rats, Sprague-Dawley
Regeneration - physiology
Short Communication
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Summary:Dermal papillae (DP) play a pivotal role in hair formation, growth and cycling. However, the number of DP is limited. In this study, we report the production of “reconstructed DP” by enclosing DP cells within an alginate–polylysine–alginate (APA) semipermeable membrane. MTT assay and electron microscopy showed that the microencapsulated dermal papilla cells retained normal activity. The microcapsules were implanted into rat footpads, which lack follicles and sebaceous glands, to assess their inductive properties. Histologic examination showed that numbers of follicle and sebaceous gland structures formed in the footpads within 6–10-week period. At the 10 weeks following transplantation, hair fibers were visible in the footpad. These findings indicate that the DP cell microcapsules retain the capacity to initiate follicle regeneration and could be considered a substitute for fresh isolated DPs.
ISSN:0340-3696
1432-069X
DOI:10.1007/s00403-008-0852-3