Platelet‐bound immunogloblins before and after platelet transfusion: measurement of in vivo binding

In routine tests to investigate immunological mechanisms as a cause for enhanced destruction of transfused platelets, serum from the patient is tested against a panel of donor lymphocytes and platelets to demonstrate the presence of antibodies against HLA or platelet specific antibodies. Here we des...

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Bibliographic Details
Published in:British journal of haematology 1999-02, Vol.104 (2), p.397-402
Main Authors: Levin, M‐D., De Vries, W., De Veld, J., Doekharan, D., Van Der Holt, B., Van 'T Veer, M. B.
Format: Article
Language:English
Subjects:
alloimmunization
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Autoimmunity
Biological and medical sciences
Blood Platelets - immunology
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Flow Cytometry
Hematologic Neoplasms - immunology
Hematologic Neoplasms - therapy
Humans
Immunoglobulin G - immunology
Medical sciences
platelet recovery
platelet transfusion
Platelet Transfusion - methods
platelet‐bound IgG
Random Allocation
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:In routine tests to investigate immunological mechanisms as a cause for enhanced destruction of transfused platelets, serum from the patient is tested against a panel of donor lymphocytes and platelets to demonstrate the presence of antibodies against HLA or platelet specific antibodies. Here we describe a flow cytometric technique in which in vivo binding of immunoglobulins (Ig) is measured. By comparing the histograms of the platelet suspensions before and after transfusion, four different patterns were obtained: no Ig binding before and after transfusion (pattern 1), pre‐existent Ig binding (patterns 2 and 3) or preferential Ig binding to the transfused platelets (pattern 4). This technique was tested in 164 random platelet transfusions and 34 HLA‐matched platelet transfusions. A statistically significant association was found between pattern 1 and a negative result of the indirect platelet immunofluorescence test for the detection of antibodies in the serum taken before the transfusion studied or a good platelet recovery and between pattern 4 and a positive indirect platelet immunofluorescence test or a low platelet recovery.
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.1999.01177.x