Immune-relevant genes expressed in rainbow trout following immunisation with a live vaccine against Ichthyophthirius multifiliis

• Published in: Diseases of aquatic organisms 2008-08, Vol.80 (3), p.189-197
• Main Authors: VON GERSDORFF JØRGENSEN, Louise, NEMLI, Egemen, HEINECKE, Rasmus D, RAIDA, Martin K, BUCHMANN, Kurt
• Format: Article
• Language: English
• Subjects: Acute-Phase Proteins - genetics; Animal aquaculture; Animal productions; Animals; Antibodies, Protozoan - blood; Biological and medical sciences; Brackish; Ciliophora - immunology; Ciliophora - pathogenicity; Ciliophora Infections - immunology; Ciliophora Infections - prevention & control; Ciliophora Infections - veterinary; Complement System Proteins - genetics; Cytokines - genetics; Fish Diseases - immunology; Fish Diseases - prevention & control; Freshwater; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - immunology; Ichthyophthirius multifiliis; Immunization - methods; Immunization - veterinary; Immunoglobulins - genetics; Injections, Intraperitoneal - veterinary; Marine; Oncorhynchus mykiss; Oncorhynchus mykiss - immunology; Oncorhynchus mykiss - parasitology; Pisciculture; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - veterinary; Protozoan Vaccines - administration & dosage; Vaccines, Attenuated - administration & dosage; Vertebrate aquaculture
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao01935

Rainbow trout Oncorhynchus mykiss were immunised by intra-peritoneal injection using a live vaccine based on Ichthyophthirius multifiliis (Ich) theronts, which previously has shown protection against white spot disease. Samples were taken pre-vaccination and on Day 1, 7, 21 and 28 post-immunisation (p.i.). Expression of immune relevant genes in the liver, spleen and head kidney was monitored by qPCR. To describe the immune reaction following this immunisation, a series of genes encoding cytokines, complement factors, immunoglobulins and acute phase reactants were studied. Genes encoding acute phase reactants in the liver were up-regulated with serum amyloid A (SAA) as the most pronounced with a 2299-fold increase at 24 h p.i. Hepcidin and pre-cerebellin were also up-regulated in the liver 24 h p.i., by 7- and 4-fold, respectively. Complement factors C3, C5 and factor B (Bf) were up-regulated in the spleen and the head kidney 24 h and 28 d p.i. Genes encoding immunoglobulins were not up-regulated, but a specific low titer IgM response (titer 25) against parasite antigens was detected by a modified ELISA 4 wk p.i.