Mouse colitis induced by Escherichia coli producing Yersinia enterocolitica 60-kilodalton heat-shock protein: Light and electron microscope study

In patients with inflammatory bowel disease (IBD), including ulcerative colitis (UC), heat-shock protein (Hsp) 60 has been detected in serum and the intestinal tract. Our mouse colitis model was established using Escherichia coli transformed with Yersinia enterocolitica Hsp60 gene as an immunizing a...

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Bibliographic Details
Published in:Digestive diseases and sciences 1999-02, Vol.44 (2), p.445-451
Main Authors: YAGITA, A, SUKEGAWA, Y, MARUYAMA, S, SATO, N, ATOMI, Y, YAMAGUCHI, H, KAMIYA, S, IHARA, T, SUGAMATA, M
Format: Article
Language:English
Subjects:
Animals
Autoantibodies - blood
Biological and medical sciences
Chaperonin 60 - genetics
Chaperonin 60 - immunology
Colitis - microbiology
Colitis - pathology
Colitis, Ulcerative - pathology
Disease Models, Animal
Escherichia coli - genetics
Gastroenterology. Liver. Pancreas. Abdomen
Intestines - ultrastructure
Medical sciences
Mice
Mice, Inbred Strains
Other diseases. Semiology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Transformation, Genetic
Yersinia enterocolitica - genetics
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Summary:In patients with inflammatory bowel disease (IBD), including ulcerative colitis (UC), heat-shock protein (Hsp) 60 has been detected in serum and the intestinal tract. Our mouse colitis model was established using Escherichia coli transformed with Yersinia enterocolitica Hsp60 gene as an immunizing antigen, and examined light and electron microscopically as compared with lesions of UC. The large intestine of mice injected with Hsp60 antigen showed swollen goblet cells, glandular dilation, erosion, ulceration, and infiltration of inflammatory cells. The change like crypt abscesses was also found, and various phases of inflammation were observed simultaneously in individual mice. In addition, the reticulum fibers were absent ultrastructurally in the subepithelial reticular layer. Hyperplasia of the thymus was found in antigen-treated mice. These lesions were similar to those of UC. These results suggest that UC-like enteritis in mice was induced by using Hsp60, considered as one of the pathogens for UC.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1026691525874