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SHOC1, an XPF Endonuclease-Related Protein, Is Essential for the Formation of Class I Meiotic Crossovers
Crossovers (COs) are essential for the completion of meiosis in most species and lead to new allelic combinations in gametes [1]. Two pathways of meiotic crossover formation have been distinguished. Class I COs, which are the major class of CO in budding yeast, mammals, Caenorhabditis elegans, and A...
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Published in: | Current biology 2008-09, Vol.18 (18), p.1432-1437 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Crossovers (COs) are essential for the completion of meiosis in most species and lead to new allelic combinations in gametes
[1]. Two pathways of meiotic crossover formation have been distinguished. Class I COs, which are the major class of CO in budding yeast, mammals,
Caenorhabditis elegans, and
Arabidopsis, depend on a group of proteins called ZMM and rely on specific DNA structure intermediates that are processed to form COs
[2–6]. We identified a novel gene,
SHOC1, involved in meiosis in
Arabidopsis.
Shoc1 mutants showed a striking reduction in the number of COs produced, a similar phenotype to the previously described
Arabidopsis zmm mutants. The early steps of recombination, revealed by DMC1 foci, and completion of synapsis are not affected in
shoc1 mutants. Double mutant analysis showed that
SHOC1 acts in the same pathway as
AtMSH5, a conserved member of the ZMM group
[7].
SHOC1 is thus a novel gene required for class I CO formation in
Arabidopsis. Sequence similarity studies detected putative SHOC1 homologs in a large range of eukaryotes including human. SHOC1 appears to be related to the XPF endonuclease protein family, which suggests that it is directly involved in the maturation of DNA intermediates that lead to COs. |
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ISSN: | 0960-9822 1879-0445 |
DOI: | 10.1016/j.cub.2008.08.041 |