Analysis of the Consensus Binding Sequence and the DNA-Binding Domain of ZF5

Murine ZF5 is a transcription factor with five zinc finger motifs that represses the c-mycgene by binding to two GC-rich elements at the promoter region. Because of its ubiquitous expression in a variety of tissues, elucidation of biological functions and cellular target genes of ZF5 is of great int...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-02, Vol.255 (2), p.528-534
Main Authors: Obata, Takuya, Yanagidani, Akifumi, Yokoro, Ken, Numoto, Michitaka, Yamamoto, Shoso
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Consensus Sequence
DNA - chemistry
DNA - metabolism
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
Mice
Nucleic Acid Heteroduplexes - chemistry
Nucleic Acid Heteroduplexes - metabolism
Oligonucleotides - chemistry
Oligonucleotides - metabolism
Peptide Fragments - chemistry
Peptide Fragments - metabolism
Repressor Proteins - chemistry
Repressor Proteins - metabolism
Zinc Fingers
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Summary:Murine ZF5 is a transcription factor with five zinc finger motifs that represses the c-mycgene by binding to two GC-rich elements at the promoter region. Because of its ubiquitous expression in a variety of tissues, elucidation of biological functions and cellular target genes of ZF5 is of great interest. As the first step of identifying cellular target genes, we have attempted to determine the consensus binding motif for ZF5. We succeeded in isolating 19 oligonucleotide duplex DNAs to which ZF5 binds and determined the binding sequences with DNase I footprinting analysis. From these sequences, we deduced the consensus binding motif for ZF5 to be GSGCGCGR. In addition, we have analyzed the DNA-binding domain of ZF5 by testing a series of deletion mutants. It turned out that the zinc fingers 3 and 4 of the five finger motifs play a critical role in DNA binding.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9970