Redox regulation of interleukin-8 expression in MKN28 cells

Recent evidence suggests a role of reactive oxygen intermediates (ROI) in intracellular signaling and regulation of gene expression. We examined whether expression of interleukin-8 (IL-8), a key cytokine in the inflammatory responses of gastric epithelial cells, is sensitive to antioxidants and oxid...

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Bibliographic Details
Published in:Digestive diseases and sciences 1999-02, Vol.44 (2), p.266-273
Main Authors: SHIMADA, T, WATANABE, N, HIRAISHI, H, TERANO, A
Format: Article
Language:English
Subjects:
Acetylcysteine - pharmacology
Biological and medical sciences
Blotting, Northern
Dimethyl Sulfoxide - pharmacology
Electrophoresis
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Gastric Mucosa - metabolism
Humans
Hydrogen Peroxide - pharmacology
Interleukin-1 - pharmacology
Interleukin-8 - biosynthesis
NF-kappa B - pharmacology
Oxidation-Reduction
Reactive Oxygen Species - metabolism
RNA, Messenger - analysis
Stomach
Transcription Factors - analysis
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - pharmacology
Vertebrates: digestive system
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Summary:Recent evidence suggests a role of reactive oxygen intermediates (ROI) in intracellular signaling and regulation of gene expression. We examined whether expression of interleukin-8 (IL-8), a key cytokine in the inflammatory responses of gastric epithelial cells, is sensitive to antioxidants and oxidative stress. IL-8 secretion was quantified by IL-8 enzyme-linked immunosorbent assay, and IL-8 mRNA expression was determined by northern blot analysis. Electrophoretic mobility shift assay was performed to detect the transcription factor, nuclear factor kappaB (NF-kappaB). N-Acetylcysteine (NAC) or dimethylsulfoxide inhibited IL-8 expression induced by tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta (IL-1beta). Externally applied H2O2 significantly up-regulated IL-8 expression. TNF-alpha-induced activation of NF-kappaB activity was suppressed by NAC, and H2O2 caused significant activation of NF-kappaB. Since ROI production is increased in the inflamed gastric mucosa, for example, in H. pylori-associated gastritis, the present results suggest that ROI may be an important modulator of IL-8 expression in gastric mucosal cells.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1026638014062