Global gene expression patterns and induction of innate immune response in human laryngeal epithelial cells in response to Acinetobacter baumannii outer membrane protein A

• Published in: FEMS immunology and medical microbiology 2008-10, Vol.54 (1), p.45-52
• Main Authors: Kim, Soon Ae, Yoo, Sung Min, Hyun, Sung Hee, Choi, Chul Hee, Yang, So Young, Kim, Hwa Jung, Jang, Byeong Churl, Suh, Seong Il, Lee, Je Chul
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii; Acinetobacter baumannii - immunology; Acinetobacter baumannii - metabolism; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Bacterial Outer Membrane Proteins - metabolism; Bacteriology; Biological and medical sciences; c-Jun protein; cDNA microarray; Cell death; Cell Line; Chemokines; Cytokines; Cytokines - genetics; Cytokines - metabolism; DNA microarrays; Epithelial cells; Epithelial Cells - immunology; Extracellular signal-regulated kinase; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Genes; Human behavior; Humans; Immune response; Immune system; Immunity, Innate - immunology; inducible nitric oxide synthase; Inflammation; Innate immunity; JNK protein; Kinases; Larynx - cytology; Larynx - immunology; MAP kinase; Membrane proteins; Membranes; Microbiology; Miscellaneous; Molecular modelling; Nitric oxide; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Nitric-oxide synthase; Oligonucleotide Array Sequence Analysis; outer membrane protein; Protein A; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; TLR2; Toll-Like Receptor 2 - genetics; Toll-Like Receptor 2 - metabolism; Toll-like receptors; Transcription factors
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2008.00446.x

The outer membrane protein A of Acinetobacter baumannii (AbOmpA) is an important pathogen-associated molecular pattern that induces host cell death. We determined the gene expression profiles of human laryngeal epithelial HEp-2 cells in response to the sublethal concentration of recombinant AbOmpA (rAbOmpA) and investigated the molecular mechanisms by which rAbOmpA induces an innate immune response. The microarray analysis showed that rAbOmpA sequentially regulated a relatively small set of genes, including those associated with signal transductions and molecules involved in immune response. Among the differentially expressed genes involved in innate immune responses, the surface expression of Toll-like receptor 2 and the production of inducible nitric oxide synthase (iNOS) were prominently observed. However, rAbOmpA did not induce the production of proinflammatory cytokines and chemokines. rAbOmpA activated c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase mitogen-activated protein kinases (MAPKs). Inhibition of JNK MAPK suppressed iNOS production in the rAbOmpA-treated HEp-2 cells. These results suggest that interaction of laryngeal epithelial cells with AbOmpA has a significant impact on the induction of innate immunity during the early stages of A. baumannii infection.