In serous ovarian neoplasms the frequency of Ki-ras mutations correlates with their malignant potential

We analysed 44 tissue samples from serous ovarian neoplasms of different malignant potential for Ki-ras mutations by denaturing gradient gel electrophoresis (DGGE) and direct sequencing after microdissection. Point mutations at codon 12 were found in 7 of 20 tumours of low malignant potential (LMP)...

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Bibliographic Details
Published in:Virchows Archiv : an international journal of pathology 1999-02, Vol.434 (2), p.117-120
Main Authors: HAAS, C. J, DIEBOLD, J, HIRSCHMANN, A, ROHRBACH, H, LÖHRS, U
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Carcinoma - genetics
Carcinoma - pathology
Cystadenoma - genetics
Cystadenoma - pathology
Female
Female genital diseases
Genes, ras
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Middle Aged
Mutation
Ovarian Neoplasms - genetics
Ovarian Neoplasms - pathology
Tumors
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Description
Summary:We analysed 44 tissue samples from serous ovarian neoplasms of different malignant potential for Ki-ras mutations by denaturing gradient gel electrophoresis (DGGE) and direct sequencing after microdissection. Point mutations at codon 12 were found in 7 of 20 tumours of low malignant potential (LMP) (35%) and in 2 of 6 well-differentiated carcinomas (33%). In contrast, no mutations were detected in the 11 poorly differentiated ovarian carcinoma samples or in the 7 serous cystadenomas. The frequency of Ki-ras mutations in serous ovarian tumours seems to correlate with the malignant potential of the neoplasms. The data favour the hypothesis of a de novo development of poorly differentiated ovarian carcinomas and do not support an evolution from LMP tumours or well-differentiated carcinomas.
ISSN:0945-6317
1432-2307
DOI:10.1007/s004280050314