Flotation method for extracting insects from poultry manure samples

We examined the efficacy of a sucrose-based flotation method for extracting 3 prevalent insect species from poultry house manure: 3rd-instar Musca domestica L. (Diptera: Muscidae), Carcinops pumilio (Erichson) (Coleoptera: Histeridae) adults, and Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrio...

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Bibliographic Details
Published in:Journal of medical entomology 1999, Vol.36 (1), p.121-123
Main Authors: Tobin, P.C, Pitts, C.W
Format: Article
Language:English
Subjects:
Alphitobius diaperinus
Analysis
Animals
Beetles
Biological and medical sciences
Carcinops pumilio
Coleoptera
extraction
flotation
Fundamental and applied biological sciences. Psychology
Histeridae
Houseflies
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Methods
Musca domestica
Poultry industry
poultry manure
Securities
sucrose flotation
Tenebrio
Tenebrionidae
Vectors. Intermediate hosts
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Summary:We examined the efficacy of a sucrose-based flotation method for extracting 3 prevalent insect species from poultry house manure: 3rd-instar Musca domestica L. (Diptera: Muscidae), Carcinops pumilio (Erichson) (Coleoptera: Histeridae) adults, and Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) adults. A 0.6 M sucrose solution in distilled water altered the specific gravity to 1.2 and caused the 3 species to float to the water surface for easy separation. An analysis of variance (ANOVA) showed significant effects of the solution volume to sample mass ratio on recovery rates (F = 35.96, P < 0.0001), but no differences in species-specific recovery rates (F = 0.97, P = 0.3824). Among samples agitated in a ratio of at least 3.2 ml solution per gram of sample mass, we observed neither significant ratio effects (F = 1.37, P = 0.2442) nor significant differences in species-specific recovery rates (F = 2.05, P = 0.1353); overall, we recovered 97.49 +/- 0.20% of all individuals of the 3 species. Our analyses demonstrated that sucrose-based flotation was a statistically valid method of collecting these species from poultry manure samples.
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/36.1.121