A reliable method for organ culture of neonatal mouse retina with long-term survival

Organ culture systems of the central nervous system have proven to be useful tools for the study of development, differentiation, and degeneration. Some studies have been limited by the inability to maintain the cultures over an extended period. Here we describe an organ culture technique for the mo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuroscience methods 1999-02, Vol.87 (1), p.57-65
Main Authors: Mosinger Ogilvie, Judith, Speck, Judith D., Lett, Jaclynn M., Fleming, Timothy T.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn - physiology
Biological and medical sciences
Cell Survival - physiology
Development
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Mice
Mice, Inbred C57BL
Microscopy, Electron
Organ culture
Organ Culture Techniques - methods
Photoreceptor degeneration
Retina - anatomy & histology
Retina - cytology
Retina - growth & development
Retinal explant, mouse
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Organ culture systems of the central nervous system have proven to be useful tools for the study of development, differentiation, and degeneration. Some studies have been limited by the inability to maintain the cultures over an extended period. Here we describe an organ culture technique for the mouse retina. This method uses commercially available supplies and reproducible procedures to maintain healthy retinas with normal architecture for 4 weeks in vitro. The system is amenable to quantitative analysis. It can be used with both normal and retinal degeneration ( rd) retinas to study of the role of various factors in photoreceptor degeneration in retinal cell fate determination and development.
ISSN:0165-0270
1872-678X
DOI:10.1016/S0165-0270(98)00157-5