Effect of cigarette smoke on endothelial regeneration in vivo and nitric oxide levels

Cigarette smoking accelerates atherosclerosis and restenosis after vascular reconstruction. The mechanisms by which smoking alters vessel structure after injury are unclear. This study examined the effects of cigarette smoking on endothelial regeneration, an important component of arterial remodelin...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of surgical research 1999-03, Vol.82 (1), p.43-47
Main Authors: SARKAR, R, GELABERT, H. A, MOHIUDDIN, K. R, THAKOR, D. K, SANTIBANEZ-GALLERANI, A. S
Format: Article
Language:English
Subjects:
Animals
Aorta, Thoracic - injuries
Aorta, Thoracic - physiology
Arteriosclerosis - etiology
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Catheterization - adverse effects
Endothelium, Vascular - injuries
Endothelium, Vascular - physiology
Humans
Male
Medical sciences
Nitric Oxide - blood
Rats
Rats, Sprague-Dawley
Regeneration - physiology
Risk Factors
Smoking - adverse effects
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cigarette smoking accelerates atherosclerosis and restenosis after vascular reconstruction. The mechanisms by which smoking alters vessel structure after injury are unclear. This study examined the effects of cigarette smoking on endothelial regeneration, an important component of arterial remodeling. Adult male rats were subjected to balloon injury of the thoracic aorta and exposed to mainstream cigarette smoke via a Griffith-type smoking machine for 2 weeks. Control groups included rats which were restrained in the machine but not smoked and a group not utilizing the machine. Aortic reendothelialization was determined using Evan's blue staining of the arterial surface. Serum levels of nitric oxide were measured to determine if smoke exposure altered this potential endothelial cell mitogen. Cigarette smoking increased aortic endothelial regeneration (78.4 +/- 4.6% vs 59.2 +/- 2.1%, P < 0.05) and was associated with an increase in serum nitric oxide level (59.9 +/- 7. 1 microM vs 28.5 +/- 1.8 microM, P < 0.05). Daily restraint alone in the smoking machine had no effect on endothelial regeneration. This is the first report on the effects of smoking on endothelial regeneration and demonstrates that smoking increases reendothelialization after large vessel injury and serum levels of nitric oxide, an EC mitogen.
ISSN:0022-4804
1095-8673
DOI:10.1006/jsre.1998.5502