Effect of electric field on liquid chromatographic separation of peptide digests: Combining capillary separation techniques

A system is described which allows operation of a range of capillary based liquid phase separations including capillary electrophoresis, isocratic and gradient capillary electrochromatography, isocratic and gradient capillary liquid chromatography and electrically assisted gradient capillary liquid...

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Bibliographic Details
Published in:Journal of Chromatography A 1999-02, Vol.832 (1), p.149-163
Main Authors: Apffel, Alex, Yin, Hongfeng, Hancock, William S, McManigill, Douglass, Frenz, John, Wu, Shiaw-Lin
Format: Article
Language:English
Subjects:
Algorithms
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid
Chromatography, Liquid
Electromagnetic Fields
Electrophoresis, Capillary
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Humans
Hydrolysis
Mass Spectrometry
Molecular Sequence Data
Peptide Mapping
Peptides
Peptides - chemistry
Peptides - isolation & purification
Proteins
Trypsin
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Summary:A system is described which allows operation of a range of capillary based liquid phase separations including capillary electrophoresis, isocratic and gradient capillary electrochromatography, isocratic and gradient capillary liquid chromatography and electrically assisted gradient capillary liquid chromatography. The system was coupled to electrospray ionization mass spectrometry in the electrically assisted capillary liquid chromatography mode to investigate the effect of applied voltage on the selectivity in peptide mapping separations. Analyses were performed on tryptic digests of recombinant human growth hormone and tissue plasminogen activator. The results show a small but useful effect on selectivity that can be used to fine tune specific separations.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00957-1