Secretion of Salmonella-specific antibodies in the oviducts of hens experimentally infected with Salmonella enteritidis

The production and secretion of Salmonella enteritidis whole cell antigen-specific antibodies in the oviducts and in the serum of laying hens experimentally infected with Salmonella enteritidis, was analyzed by ELISA. The dynamics of the antibody levels in the oviducts were identical to that in the...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1999-02, Vol.67 (2), p.185-193
Main Authors: Withanage, G.S.K, Sasai, K, Fukata, T, Miyamoto, T, Baba, E
Format: Article
Language:English
Subjects:
Animals
ANTIBODIES
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - metabolism
Antibody
ANTICORPS
ANTICUERPOS
Chicken
Chickens - metabolism
Chickens - microbiology
ELISA
Enzyme-Linked Immunosorbent Assay - veterinary
EXPERIMENTAL INFECTION
Female
GALLINA PONEDORA
IMMUNITE
IMMUNITY
INFECCION EXPERIMENTAL
INFECTION EXPERIMENTALE
INMUNIDAD
LAYER CHICKENS
Local immunity
OVIDUCTE
OVIDUCTOS
OVIDUCTS
Oviducts - immunology
Oviducts - metabolism
POULE PONDEUSE
Poultry Diseases - immunology
Salmonella
SALMONELLA ENTERITIDIS
Salmonella enteritidis - immunology
Salmonella Infections, Animal - immunology
TEST ELISA
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Summary:The production and secretion of Salmonella enteritidis whole cell antigen-specific antibodies in the oviducts and in the serum of laying hens experimentally infected with Salmonella enteritidis, was analyzed by ELISA. The dynamics of the antibody levels in the oviducts were identical to that in the serum. Subclasses of antibodies (IgA, IgG, and IgM) in the infected hens were found to increase significantly ( p < 0.01) compared to those in the control uninfected hens throughout the experiment. IgG and IgM levels in both oviducts and in sera reached to a peak by 14 days post-inoculation, and remained elevated throughout. The secretion of IgA seemed to be transient since the IgA levels increased to a peak 7 days after both primary and secondary inoculations, and declined rapidly. The elevated levels of antibodies were followed by partial clearance of Salmonella organisms from the oviducts. The present results indicate a significant local immune reaction against the Salmonella infection and suggest an association of the local antibodies with the clearance of Salmonella from the oviducts at least partially.
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(98)00223-2