Banana bunchy top virus DNA-2 to 6 are monocistronic

Banana bunchy top virus (BBTV) DNA-3 to 6 have each previously been shown to contain one large open reading frame in the virion sense, whereas no large ORF had been identified in BBTV DNA-2. RNAs transcribed from the BBTV genome were mapped using northern hybridisation and 3' RACE. One mRNA was...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 1999, Vol.144 (1), p.89-105
Main Authors: Beetham, P.R, Harding, R.M, Dale, J.L
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
Amino Acid Sequence
Base Sequence
Biological and medical sciences
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Genetics
Genome, Viral
genomics
messenger RNA
Microbiology
Molecular Sequence Data
nucleotide sequences
Open Reading Frames - genetics
Pentalonia nigronervosa
Phytopathology. Animal pests. Plant and forest protection
Plant Viruses - genetics
Plant viruses and viroids
RNA, Viral - genetics
Sequence Alignment
Systematics. Structure, properties and multiplication. Genetics
Virology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Banana bunchy top virus (BBTV) DNA-3 to 6 have each previously been shown to contain one large open reading frame in the virion sense, whereas no large ORF had been identified in BBTV DNA-2. RNAs transcribed from the BBTV genome were mapped using northern hybridisation and 3' RACE. One mRNA was transcribed from each of BBTV DNA-2 to 6 and four of these mRNAs mapped to the ORFs previously identified in BBTV DNA-3 to 6. The mRNA of BBTV DNA-2 was transcribed from a virion sense ORF probably using a TATA box sequence different to that in BBTV DNA-1, and DNA-3 to 6. This ORF encoded a 10 kDa protein of unknown function. The 3' untranslated region of the five mRNAs varied from 25 nucleotides (BBTV DNA-6) to 167 nucleotides (BBTV DNA-4) and each contained putative polyadenylation signals with associated GT rich sequence together with a possible termination signal (C/T/A)TGTAA conserved in all five mRNAs.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050487