Identification of a Drosophila homologue to vertebrate Crk by interaction with MBC
The vertebrate adapter protein termed Crk was initially identified from the chicken CT10 retrovirus on the basis of its transforming activity (Mayer et al., 1988. Nature 332, 272–275). We have identified a Drosophila protein with homology to vertebrate Crk, termed dCRK, by interaction with the prote...
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Published in: | Gene 1999-03, Vol.228 (1), p.243-252 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The vertebrate adapter protein termed Crk was initially identified from the chicken CT10 retrovirus on the basis of its transforming activity (Mayer et al., 1988. Nature 332, 272–275). We have identified a
Drosophila protein with homology to vertebrate Crk, termed dCRK, by interaction with the protein encoded by the
Drosophila myoblast city (
mbc) gene. The dCRK protein has extensive homology to the both the Crk-II form of vertebrate Crk and the Crk-related protein CRKL, and includes one SH2 domain followed by two SH3 domains. A single protein of approx. 37
kDa is detected in extracts from embryos, and Northern analysis revealed a single transcript of 1.3
kb. The
dCrk mRNA is abundant throughout embryogenesis, declines during the larval stages, and reappears during pupation. In addition, maternally-provided transcripts have been detected. During embryogenesis, the spatial distribution of this transcript is relatively broad and appears to include all germ layers. Finally,
dCrk is located on the fourth chromosome, approximately at cytological position 101F-102A. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(99)00010-4 |