The B Subunit of the CAAT-binding Factor NFY Binds the Central Segment of the Co-activator p300

• Published in: The Journal of biological chemistry 1999-03, Vol.274 (12), p.7623-7626
• Main Authors: Faniello, M C, Bevilacqua, M A, Condorelli, G, de Crombrugghe, B, Maity, S N, Avvedimento, V E, Cimino, F, Costanzo, F
• Format: Article
• Language: English
• Subjects: Binding Sites; CCAAT-Enhancer-Binding Proteins; DNA-Binding Proteins - metabolism; Ferritins - genetics; HeLa Cells; Humans; Nuclear Proteins - metabolism; Promoter Regions, Genetic; Protein Conformation; Trans-Activators - metabolism
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.274.12.7623

We report that the heterotrimeric transcription factor NFY or “CAAT-binding factor” binds the −60 region of the human H ferritin promoter, the B site. DNA binding analysis with specific antibodies demonstrates that NFY/B/C subunits tightly bind this site and that NFY/C subunit is masked in vivo by binding with other protein(s). NFY binds the co-activator p300. Specifically, the NFY/B subunit interacts with the central segment of p300 in vivo and in vitro . cAMP substantially increases the formation of the NFY·p300 complex. Taken together these data provide a general model of cAMP induction of non-CRE-containing promoters and suggest that the NFY-B·p300 complex is located at the 5′ end of the promoter and the NFY-B·C·TFIIB on the 3′ end toward the transcription start site.