Improved method of determining thiamylal enantiomers in human serum by high-performance liquid chromatography

Thiamylal, a widely used anesthetic drug, has two enantiomers. We developed a simple and rapid method for measuring the thiamylal enantiomers in human serum. The method involves a liquid–liquid extraction procedure followed by chiral resolution using a 5 μm silica-bonded α 1-acid glycoprotein column...

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Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1999-02, Vol.723 (1), p.307-311
Main Authors: Sueyasu, Masanori, Fujito, Kimihito, Makino, Kazutaka, Shuto, Hideki, Kataoka, Yasufumi, Oishi, Ryozo
Format: Article
Language:English
Subjects:
Analysis
Anesthetics, Intravenous - blood
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Humans
Hypnotics and Sedatives - blood
Medical sciences
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Stereoisomerism
Thiamylal
Thiamylal - blood
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cited_by cdi_FETCH-LOGICAL-c390t-4fe744a78d94c540de661b9932f676ec8d106e0aae3108f8504978706f8efec03
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container_title Journal of chromatography. B, Biomedical sciences and applications
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creator Sueyasu, Masanori
Fujito, Kimihito
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description Thiamylal, a widely used anesthetic drug, has two enantiomers. We developed a simple and rapid method for measuring the thiamylal enantiomers in human serum. The method involves a liquid–liquid extraction procedure followed by chiral resolution using a 5 μm silica-bonded α 1-acid glycoprotein column (Chiral-AGP). The thiamylal enantiomers and internal standard were eluted within 15 min and were well-resolved. At concentrations of 1, 5 and 20 μg ml −1, the relative standard deviations of R(+)- and S(−)-thiamylal were 1.35–2.88% and 1.37–3.01%, respectively, for the intra-day assay, and 2.93–4.46% and 2.46–4.84%, respectively, for the inter-day assay. This method facilitates the routine monitoring and pharmacokinetic studies of thiamylal enantiomers.
doi_str_mv 10.1016/S0378-4347(98)00496-4
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subjects Analysis
Anesthetics, Intravenous - blood
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Humans
Hypnotics and Sedatives - blood
Medical sciences
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Stereoisomerism
Thiamylal
Thiamylal - blood
title Improved method of determining thiamylal enantiomers in human serum by high-performance liquid chromatography
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