G10.3 monoclonal antibody identifies novel functional cell surface structures expressed by normal B lymphocytes and various malignant cell lines

: G10.3, a unique monoclonal antibody (mAb), was produced to better characterize lymphocyte subsets. In the present study, we show that this mAb identifies 118, 83 and 51 kDa cell surface sialylated glycoproteins on the immunizing cell line YTindi. The reactivity of G10.3 mAb is restricted in normal...

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Bibliographic Details
Published in:Tissue antigens 1999-01, Vol.53 (1), p.23-32
Main Authors: Schiavon, V., Elhabazi, A., Agrawal, S., Tawab, A., Nikolova, M., Boumsell, L., Bensussan, A.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antigens, Neoplasm - immunology
Antigens, Surface - immunology
Apoptosis
B lymphocyte antigen
B-Lymphocytes - immunology
Cell Line, Transformed - immunology
Cytotoxicity, Immunologic
Epitopes - immunology
Fluorescent Antibody Technique, Indirect
Herpesvirus 4, Human
Humans
Hybridomas - immunology
Leukemia - immunology
Leukemia - pathology
Lymphocyte Culture Test, Mixed
Lymphocyte Subsets - immunology
Membrane Glycoproteins - immunology
Molecular Weight
monoclonal antibody
N-Acetylneuraminic Acid - analysis
Neoplasm Proteins - immunology
Neoplastic Stem Cells - immunology
Precipitin Tests
programmed cell death
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Summary:: G10.3, a unique monoclonal antibody (mAb), was produced to better characterize lymphocyte subsets. In the present study, we show that this mAb identifies 118, 83 and 51 kDa cell surface sialylated glycoproteins on the immunizing cell line YTindi. The reactivity of G10.3 mAb is restricted in normal cells to B lymphocytes, whereas within tumoral cell lines various lymphoid and non‐lymphoid cells were found positive. Interestingly, functional studies revealed that triggering G10.3 mAb reactive molecules with soluble antibody led to an inhibition of growth and to an induction of programmed cell death in tumor cell lines expressing high levels of reactive molecules.
ISSN:0001-2815
1399-0039
DOI:10.1034/j.1399-0039.1999.530103.x