Identification of Progesterone-Dependent Messenger Ribonucleic Acid Regulatory Patterns in the Rhesus Monkey Endometrium by Differential-Display Reverse Transcription-Polymerase Chain Reaction

• Published in: Biology of reproduction 1999-04, Vol.60 (4), p.1029-1035
• Main Authors: ACE, C. I, OKULICZ, W. C
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; DNA, Complementary - analysis; Endometrium - metabolism; Estrogens - genetics; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Macaca mulatta; Mammalian female genital system; Menstrual Cycle; Morphology. Physiology; Progesterone - genetics; Progesterone - pharmacology; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - metabolism; Vertebrates: reproduction
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod60.4.1029

We used differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) to identify different patterns of progesterone (P 4 )-dependent gene regulation in rhesus monkey endometria. Complementary DNA populations representing the proliferative phase (estrogen dominant, EcDNA) and an inadequate secretory phase (low level of P 4 , IcDNA) were compared with a cDNA population representing an adequate secretory phase (normal level of P 4 , PcDNA). We were able to distinguish four different levels of mRNA regulation: 1) up-regulation by P 4 during an adequate secretory phase, 2) autologous down-regulation (IcDNA versus PcDNA), 3) lower abundance in IcDNA compared to PcDNA, and 4) P 4 -dependent inhibition of EcDNA gene expression. We isolated and sequenced 16 fragments representing these different levels of P 4 regulation. The sequence of three fragments that were autologously down-regulated (I1, I2, I4) matched previously entered GenBank mRNAs: I1 encodes serine/threonine protein phosphatase A; I2 encodes oxobutanoate dehydrogenase E1b-beta; and I4 encodes line-1 reverse transcriptase homologue. Six other fragments exhibited homology to uncharacterized expressed sequence tags, sequence site tags, and cosmid clones. The remaining seven fragments exhibited no significant homology to GenBank entries at this time. The various patterns of P 4 -dependent gene regulation identified in the present study are likely to play roles in the temporal orchestration of events that lead to proper maturation of the endometrium.