Surfaces Designed to Control the Projected Area and Shape of Individual Cells

Materials with spatially resolved surface chemistry were designed to isolate individual mammalian cells to determine the influence of projected area on specific cell functions (e.g., proliferation, cytoskeletal organization). Surfaces were fabricated using a photolithographic process resulting in is...

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Bibliographic Details
Published in:Journal of biomechanical engineering 1999-02, Vol.121 (1), p.40-48
Main Authors: Thomas, C. H, Lhoest, J.-B, Castner, D. G, McFarland, C. D, Healy, K. E
Format: Article
Language:English
Subjects:
Actins - analysis
Adhesion
Adsorption
Animals
Biocompatible Materials
Biological and medical sciences
Blood Proteins - pharmacokinetics
Cell Adhesion
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell cultures. Hybridization. Fusion
Cytoskeleton
Equipment Design
Fundamental and applied biological sciences. Psychology
General aspects
Humans
Hydrogels
Interpenetrating polymer networks
Materials Testing
Molecular and cellular biology
Osteoarthritis, Hip - pathology
Parietal Bone - cytology
Photolithography
Quartz
Rats
Rats, Sprague-Dawley
Silanes
Skull - cytology
Surface chemistry
Surface Properties
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Summary:Materials with spatially resolved surface chemistry were designed to isolate individual mammalian cells to determine the influence of projected area on specific cell functions (e.g., proliferation, cytoskeletal organization). Surfaces were fabricated using a photolithographic process resulting in islands of cell binding N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS) separated by a nonadhesive interpenetrating polymer network [poly (acrylamide-co-ethylene glycol); P(AAm-co-EG)]. The surfaces contained over 3800 adhesive islands/cm2, allowing for isolation of single cells with projected areas ranging from 100 μm2 to 10,000 μm2. These surfaces provide a useful tool for researching how cell morphology and mechanical forces affect cell function.
ISSN:0148-0731
1528-8951
DOI:10.1115/1.2798041