chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix

• Published in: Insect molecular biology 2008-10, Vol.17 (5), p.573-585
• Main Authors: Toprak, U, Baldwin, D, Erlandson, M, Gillott, C, Hou, X, Coutu, C, Hegedus, D.D
• Format: Article
• Language: English
• Subjects: Amidohydrolases - chemistry; Amidohydrolases - genetics; Amidohydrolases - metabolism; Amino Acid Sequence; Animals; chitin deacetylase; Chromatography, Liquid; Databases, Genetic; Electrophoresis, Gel, Two-Dimensional; Expressed Sequence Tags; Gene Expression Regulation, Enzymologic; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Intestines - enzymology; Lepidoptera; Lepidoptera - enzymology; Lepidoptera - genetics; lipase; Lipase - chemistry; Lipase - genetics; Lipase - metabolism; Mamestra configurata; Mass Spectrometry; Molecular Sequence Data; Noctuidae; peritrophic matrix; peritrophic membrane; peritrophin; Phylogeny; Protein Transport; Recombinant Proteins - metabolism; Sequence Alignment; triacylglycerol lipase
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1111/j.1365-2583.2008.00829.x

One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.