Loading…
chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix
One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armywo...
Saved in:
Published in: | Insect molecular biology 2008-10, Vol.17 (5), p.573-585 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3 |
---|---|
cites | cdi_FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3 |
container_end_page | 585 |
container_issue | 5 |
container_start_page | 573 |
container_title | Insect molecular biology |
container_volume | 17 |
creator | Toprak, U Baldwin, D Erlandson, M Gillott, C Hou, X Coutu, C Hegedus, D.D |
description | One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult. |
doi_str_mv | 10.1111/j.1365-2583.2008.00829.x |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69633803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69633803</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3</originalsourceid><addsrcrecordid>eNqNkctuEzEUhkcIREvhFcBigWAxwWOPb4gNVBAq0gKCAjvLHR83DnPD9kDyFjwyThMViQ1Yso6l8_1Htr-iQBWeVXk9Xc0qyllJmKQzgrGc5U3UbH2jOLxu3CwOseKkrLBgB8WdGFc4U4qr28VBJSWRsqaHxa9m6ZPvkQXTQNq0JgIyvUXjlEzyPwD5PkKTckkQM2ha1PoxUxGZAKgZunHooU8RDQ6lJaBT02UwmNzqnb-cgkkGPV7A6O0wJgjmGTobmjR5a-AJGiH4FIZx6RvUmRT8-m5xy5k2wr19PSrOX7_6dPymXLybnxy_WJQNY0SVVDhCBcaOg6qIBWJrKqSoeZMfQrngzlnlQColjHSUudpiIRhQe8GErS09Kh7t5o5h-D7lK-vOxwba1vQwTFFzxSmVmP4TJLiuuSB1Bh_-Ba6GKeQfu2IEoZKpDMkd1IQhxgBOj8F3Jmx0hfXWrV7prUK9Vai3bvWVW73O0fv7-dNFB_ZPcC8zA893wE_fwua_B-uT05f5kOPlLu5jgvV13IRvmgsqmP5yNtf8_fwt-fC50l8z_2DHOzNocxl81OcfCa4orhhVghH6G_3AzTk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>204723859</pqid></control><display><type>article</type><title>chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Toprak, U ; Baldwin, D ; Erlandson, M ; Gillott, C ; Hou, X ; Coutu, C ; Hegedus, D.D</creator><creatorcontrib>Toprak, U ; Baldwin, D ; Erlandson, M ; Gillott, C ; Hou, X ; Coutu, C ; Hegedus, D.D</creatorcontrib><description>One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/j.1365-2583.2008.00829.x</identifier><identifier>PMID: 18828843</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Amidohydrolases - chemistry ; Amidohydrolases - genetics ; Amidohydrolases - metabolism ; Amino Acid Sequence ; Animals ; chitin deacetylase ; Chromatography, Liquid ; Databases, Genetic ; Electrophoresis, Gel, Two-Dimensional ; Expressed Sequence Tags ; Gene Expression Regulation, Enzymologic ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Intestines - enzymology ; Lepidoptera ; Lepidoptera - enzymology ; Lepidoptera - genetics ; lipase ; Lipase - chemistry ; Lipase - genetics ; Lipase - metabolism ; Mamestra configurata ; Mass Spectrometry ; Molecular Sequence Data ; Noctuidae ; peritrophic matrix ; peritrophic membrane ; peritrophin ; Phylogeny ; Protein Transport ; Recombinant Proteins - metabolism ; Sequence Alignment ; triacylglycerol lipase</subject><ispartof>Insect molecular biology, 2008-10, Vol.17 (5), p.573-585</ispartof><rights>No claims to original government works. Journal compilation © 2008 The Royal Entomological Society</rights><rights>Journal compilation © 2008 Royal Entomological Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3</citedby><cites>FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18828843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toprak, U</creatorcontrib><creatorcontrib>Baldwin, D</creatorcontrib><creatorcontrib>Erlandson, M</creatorcontrib><creatorcontrib>Gillott, C</creatorcontrib><creatorcontrib>Hou, X</creatorcontrib><creatorcontrib>Coutu, C</creatorcontrib><creatorcontrib>Hegedus, D.D</creatorcontrib><title>chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.</description><subject>Amidohydrolases - chemistry</subject><subject>Amidohydrolases - genetics</subject><subject>Amidohydrolases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>chitin deacetylase</subject><subject>Chromatography, Liquid</subject><subject>Databases, Genetic</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Expressed Sequence Tags</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Intestines - enzymology</subject><subject>Lepidoptera</subject><subject>Lepidoptera - enzymology</subject><subject>Lepidoptera - genetics</subject><subject>lipase</subject><subject>Lipase - chemistry</subject><subject>Lipase - genetics</subject><subject>Lipase - metabolism</subject><subject>Mamestra configurata</subject><subject>Mass Spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Noctuidae</subject><subject>peritrophic matrix</subject><subject>peritrophic membrane</subject><subject>peritrophin</subject><subject>Phylogeny</subject><subject>Protein Transport</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Alignment</subject><subject>triacylglycerol lipase</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqNkctuEzEUhkcIREvhFcBigWAxwWOPb4gNVBAq0gKCAjvLHR83DnPD9kDyFjwyThMViQ1Yso6l8_1Htr-iQBWeVXk9Xc0qyllJmKQzgrGc5U3UbH2jOLxu3CwOseKkrLBgB8WdGFc4U4qr28VBJSWRsqaHxa9m6ZPvkQXTQNq0JgIyvUXjlEzyPwD5PkKTckkQM2ha1PoxUxGZAKgZunHooU8RDQ6lJaBT02UwmNzqnb-cgkkGPV7A6O0wJgjmGTobmjR5a-AJGiH4FIZx6RvUmRT8-m5xy5k2wr19PSrOX7_6dPymXLybnxy_WJQNY0SVVDhCBcaOg6qIBWJrKqSoeZMfQrngzlnlQColjHSUudpiIRhQe8GErS09Kh7t5o5h-D7lK-vOxwba1vQwTFFzxSmVmP4TJLiuuSB1Bh_-Ba6GKeQfu2IEoZKpDMkd1IQhxgBOj8F3Jmx0hfXWrV7prUK9Vai3bvWVW73O0fv7-dNFB_ZPcC8zA893wE_fwua_B-uT05f5kOPlLu5jgvV13IRvmgsqmP5yNtf8_fwt-fC50l8z_2DHOzNocxl81OcfCa4orhhVghH6G_3AzTk</recordid><startdate>200810</startdate><enddate>200810</enddate><creator>Toprak, U</creator><creator>Baldwin, D</creator><creator>Erlandson, M</creator><creator>Gillott, C</creator><creator>Hou, X</creator><creator>Coutu, C</creator><creator>Hegedus, D.D</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200810</creationdate><title>chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix</title><author>Toprak, U ; Baldwin, D ; Erlandson, M ; Gillott, C ; Hou, X ; Coutu, C ; Hegedus, D.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amidohydrolases - chemistry</topic><topic>Amidohydrolases - genetics</topic><topic>Amidohydrolases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>chitin deacetylase</topic><topic>Chromatography, Liquid</topic><topic>Databases, Genetic</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Expressed Sequence Tags</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Intestines - enzymology</topic><topic>Lepidoptera</topic><topic>Lepidoptera - enzymology</topic><topic>Lepidoptera - genetics</topic><topic>lipase</topic><topic>Lipase - chemistry</topic><topic>Lipase - genetics</topic><topic>Lipase - metabolism</topic><topic>Mamestra configurata</topic><topic>Mass Spectrometry</topic><topic>Molecular Sequence Data</topic><topic>Noctuidae</topic><topic>peritrophic matrix</topic><topic>peritrophic membrane</topic><topic>peritrophin</topic><topic>Phylogeny</topic><topic>Protein Transport</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Alignment</topic><topic>triacylglycerol lipase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toprak, U</creatorcontrib><creatorcontrib>Baldwin, D</creatorcontrib><creatorcontrib>Erlandson, M</creatorcontrib><creatorcontrib>Gillott, C</creatorcontrib><creatorcontrib>Hou, X</creatorcontrib><creatorcontrib>Coutu, C</creatorcontrib><creatorcontrib>Hegedus, D.D</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toprak, U</au><au>Baldwin, D</au><au>Erlandson, M</au><au>Gillott, C</au><au>Hou, X</au><au>Coutu, C</au><au>Hegedus, D.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2008-10</date><risdate>2008</risdate><volume>17</volume><issue>5</issue><spage>573</spage><epage>585</epage><pages>573-585</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18828843</pmid><doi>10.1111/j.1365-2583.2008.00829.x</doi><tpages>13</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0962-1075 |
ispartof | Insect molecular biology, 2008-10, Vol.17 (5), p.573-585 |
issn | 0962-1075 1365-2583 |
language | eng |
recordid | cdi_proquest_miscellaneous_69633803 |
source | Wiley-Blackwell Read & Publish Collection |
subjects | Amidohydrolases - chemistry Amidohydrolases - genetics Amidohydrolases - metabolism Amino Acid Sequence Animals chitin deacetylase Chromatography, Liquid Databases, Genetic Electrophoresis, Gel, Two-Dimensional Expressed Sequence Tags Gene Expression Regulation, Enzymologic Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Intestines - enzymology Lepidoptera Lepidoptera - enzymology Lepidoptera - genetics lipase Lipase - chemistry Lipase - genetics Lipase - metabolism Mamestra configurata Mass Spectrometry Molecular Sequence Data Noctuidae peritrophic matrix peritrophic membrane peritrophin Phylogeny Protein Transport Recombinant Proteins - metabolism Sequence Alignment triacylglycerol lipase |
title | chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T11%3A13%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=chitin%20deacetylase%20and%20putative%20insect%20intestinal%20lipases%20are%20components%20of%20the%20Mamestra%20configurata%20(Lepidoptera:%20Noctuidae)%20peritrophic%20matrix&rft.jtitle=Insect%20molecular%20biology&rft.au=Toprak,%20U&rft.date=2008-10&rft.volume=17&rft.issue=5&rft.spage=573&rft.epage=585&rft.pages=573-585&rft.issn=0962-1075&rft.eissn=1365-2583&rft_id=info:doi/10.1111/j.1365-2583.2008.00829.x&rft_dat=%3Cproquest_cross%3E69633803%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5529-37f23700f6e912de2d4378746ceac3676ffd9fe8997a8f35f4d0775e3db57d4d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=204723859&rft_id=info:pmid/18828843&rfr_iscdi=true |