Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

• Published in: American journal of physiology. Lung cellular and molecular physiology 2008-10, Vol.295 (4), p.L670-L679
• Main Authors: Vieira, Rodolfo P, de Andrade, Vanessa F, Duarte, Anna Cecilia S, dos Santos, Angela B. G, Mauad, Thais, Martins, Milton A, Dolhnikoff, Marisa, Carvalho, Celso R. F
• Format: Article
• Language: English
• Subjects: Anaerobiosis; Animals; Asthma; Conditioning (Psychology); Cytokines; Exercise Test; Hypersensitivity - pathology; Hypersensitivity - physiopathology; Lung - pathology; Lung - physiology; Lung - physiopathology; Lungs; Male; Mice; Mice, Inbred BALB C; Ovalbumin - immunology; Pneumonia - pathology; Pneumonia - physiopathology; Pulmonary arteries; Pulmonary Circulation - physiology; Reference Values; Rodents; Time Factors; Tissues
• ISSN: 1040-0605; 1522-1504
• DOI: 10.1152/ajplung.00465.2007

Departments of 1 Pathology, 2 Physical Therapy, and 3 Medicine, School of Medicine, University of São Paulo, São Paulo, Brazil Submitted 8 November 2007 ; accepted in final form 14 August 2008 Recent evidence suggests that asthma leads to inflammation and remodeling not only in the airways but also in pulmonary vessels and parenchyma. In addition, some studies demonstrated that aerobic training decreases chronic allergic inflammation in the airways; however, its effects on the pulmonary vessels and parenchyma have not been previously evaluated. Our objective was to test the hypothesis that aerobic conditioning reduces inflammation and remodeling in pulmonary vessels and parenchyma in a model of chronic allergic lung inflammation. Balb/c mice were sensitized at days 0 , 14 , 28 , and 42 and challenged with ovalbumin (OVA) from day 21 to day 50 . Aerobic training started on day 21 and continued until day 50 . Pulmonary vessel and parenchyma inflammation and remodeling were evaluated by quantitative analysis of eosinophils and mononuclear cells and by collagen and elastin contents and smooth muscle thickness. Immunohistochemistry was performed to quantify the density of positive cells to interleukin (IL)-2, IL-4, IL-5, interferon- , IL-10, monocyte chemotatic protein (MCP)-1, nuclear factor (NF)- B p65, and insulin-like growth factor (IGF)-I. OVA exposure induced pulmonary blood vessels and parenchyma inflammation as well as increased expression of IL-4, IL-5, MCP-1, NF- B p65, and IGF-I by inflammatory cells were reduced by aerobic conditioning. OVA exposure also induced an increase in smooth muscle thickness and elastic and collagen contents in pulmonary vessels, which were reduced by aerobic conditioning. Aerobic conditioning increased the expression of IL-10 in sensitized mice. We conclude that aerobic conditioning decreases pulmonary vascular and parenchymal inflammation and remodeling in this experimental model of chronic allergic lung inflammation in mice. asthma; aerobic training; cytokines; pulmonary inflammation; pulmonary remodeling Address for reprint requests and other correspondence: C. R. Fernandes de Carvalho, School of Medicine, Univ. of São Paulo, Av. Dr. Arnaldo, 455, 1° andar, sala 1216, 01246-903, São Paulo, SP, Brazil (e-mail: cscarval{at}usp.br )