pPAC-ResQ: A Yeast–Bacterial Shuttle Vector for Capturing Inserts from P1 and PAC Clones by Recombinogenic Targeted Cloning

We have developed a method to capture inserts from P1 and P1 artificial chromosome (PAC) clones into a yeast–bacteria shuttle vector by using recombinogenic targeting. We have engineered a vector, pPAC-ResQ, a derivative of pClasper, which was previously used to capture inserts from yeast artificial...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1999-03, Vol.56 (3), p.337-339
Main Authors: Bhargava, Jaya, Shashikant, Cooduvalli S., Carr, Janet L., Bentley, Kevin L., Amemiya, Chris T., Ruddle, Frank H.
Format: Article
Language:English
Subjects:
Bacteria - genetics
Biological and medical sciences
Cloning, Molecular - methods
Diverse techniques
Escherichia coli
Fundamental and applied biological sciences. Psychology
Gene Library
Genetic Vectors - genetics
Models, Biological
Molecular and cellular biology
Molecular Sequence Data
Yeasts - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a method to capture inserts from P1 and P1 artificial chromosome (PAC) clones into a yeast–bacteria shuttle vector by using recombinogenic targeting. We have engineered a vector, pPAC-ResQ, a derivative of pClasper, which was previously used to capture inserts from yeast artificial chromosome clones. pPAC-ResQ contains DNA fragments flanking the inserts in P1 and PAC vectors as recombinogenic ends. When linearized pPAC-ResQ vector and P1 or PAC DNA are cotransformed into yeast, recombination between the two leads to the transfer of inserts into pPAC-ResQ. pPAC-ResQ clones thus obtained can be further modified in yeast for functional analysis and shuttled toEscherichia colito produce large quantities of cloned DNA. This approach provides a rapid method to modify P1/PAC clones for functional analysis.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1998.5710