Quantitation of argyrophilic nucleolar organizer regions in regenerating muscle fibers in Duchenne and Becker muscular dystrophies and polymyositis

We have investigated the quantity of argyrophilic nucleolar organizer region (AgNOR) proteins in vastus lateralis muscle samples from 13 patients with Duchenne muscular dystrophy (DMD) (6 months-12 years), 9 with Becker muscular dystrophy (BMD) (13 months-36 years), 9 with polymyositis (PM) (8-77 ye...

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Bibliographic Details
Published in:Acta neuropathologica 1999-03, Vol.97 (3), p.247-252
Main Authors: TUCCARI, G, GIUFFRE, G, CRISAFULLI, C, MONICI, M. C, TOSCANO, A, VITA, G
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Becker's muscular dystrophy
Biological and medical sciences
Biopsy
Cell Nucleus - metabolism
Child
Child, Preschool
Diseases of striated muscles. Neuromuscular diseases
Duchenne's muscular dystrophy
Female
Humans
Image Processing, Computer-Assisted
Infant
Male
Medical sciences
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal - metabolism
Muscle, Skeletal - pathology
Muscular Dystrophies - metabolism
Muscular dystrophy
Neurology
Nuclei
Nucleoli
Nucleolus Organizer Region - metabolism
Polymyositis
Polymyositis - metabolism
Protein biosynthesis
Quantitation
Regeneration - physiology
Silver Staining
Statistical analysis
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Summary:We have investigated the quantity of argyrophilic nucleolar organizer region (AgNOR) proteins in vastus lateralis muscle samples from 13 patients with Duchenne muscular dystrophy (DMD) (6 months-12 years), 9 with Becker muscular dystrophy (BMD) (13 months-36 years), 9 with polymyositis (PM) (8-77 years) and 10 normal subjects (5 months-32 years). AgNORs were visualized on 4-microm-thick cryostat sections and quantified according to the guidelines of the Committee on AgNOR Quantitation; statistical analysis was performed on the mean AgNOR area (NORA) values. The mean NORA values encountered in DMD (4.327+/-0.791 microm2), BMD (3.534+/-0.312 microm2) and PM (3.785+/-0.424 microm2) samples were significantly (P
ISSN:0001-6322
1432-0533
DOI:10.1007/s004010050981