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The complete genome sequence of the major component of a mild citrus tristeza virus isolate
MC Vives, L Rubio, C Lopez, J Navas-Castillo, MR Albiach-Marti, WO Dawson, J Guerri, R Flores and P Moreno Instituto Valenciano de Investigaciones Agrarias, Moncada, Spain. The genome of the Spanish mild isolate T385 of citrus tristeza virus (CTV) was completely sequenced and compared with the genom...
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Published in: | Journal of general virology 1999-03, Vol.80 (3), p.811-816 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
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Summary: | MC Vives, L Rubio, C Lopez, J Navas-Castillo, MR Albiach-Marti, WO Dawson, J Guerri, R Flores and P Moreno
Instituto Valenciano de Investigaciones Agrarias, Moncada, Spain.
The genome of the Spanish mild isolate T385 of citrus tristeza virus (CTV)
was completely sequenced and compared with the genomes of the severe
isolates T36 (Florida), VT (Israel) and SY568 (California). The genome of
T385 was 19,259 nt in length, 37 nt shorter than the genome of T36, and 33
and 10 nt longer than those of VT and SY568, respectively, but their
organization was identical. T385 had mean nucleotide identities of 81.3,
89.3 and 94% with T36, VT and SY568, respectively. The 3' UTR had over 97%
identity in all isolates, whereas the 5' UTR of T385 had 67% identity with
VT, 66.3% with SY568 and only 42.5% with T36. In the coding regions, the
nucleotide differences between T385 and VT were evenly distributed along
the genome (around 90% identity); this was not observed between T385 and
the other isolates. T385 and T36 had nucleotide identities around 90% in
the eight 3'-terminal ORFs of the genome, but only 72.3% in ORF 1a, a
divergence pattern similar to that reported previously for T36 and VT. T385
and SY568 had nucleotide identities close to 90% in the 5'- and 3'-
terminal regions of the genome, whereas the central region had over 99%
identity. Our data suggest that the central region in the SY568 genome
results from RNA recombination between two CTV genomes, one of which was
almost identical to T385. |
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ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-80-3-811 |